StemMACS™ eGFP mRNA encodes enhanced green fluorescent protein (eGFP) for transient transfection.

Data and images for StemMACS™ eGFP mRNA

Figures

Figure 1

eGFP expression after mRNA transfection:
Human BJ fibroblasts were transfected with 150 ng StemMACS™ eGFP mRNA. 24 hours after transfection, eGFP expression (green) and cytoplamic localization (DAPI counterstain) were analyzed by immunofluorescence microscopy.
Untransfected cells
eGFP-tranfected cells
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Figure 1

eGFP expression after mRNA transfection:
Human BJ fibroblasts were transfected with 150 ng StemMACS™ eGFP mRNA. 24 hours after transfection, eGFP expression (green) and cytoplamic localization (DAPI counterstain) were analyzed by immunofluorescence microscopy.
View details

Figure 1

eGFP expression after mRNA transfection:
Human BJ fibroblasts were transfected with 150 ng StemMACS™ eGFP mRNA. 24 hours after transfection, eGFP expression (green) and cytoplamic localization (DAPI counterstain) were analyzed by immunofluorescence microscopy.

Specifications for StemMACS™ eGFP mRNA

Overview

StemMACS™ eGFP mRNA encodes enhanced green fluorescent protein (eGFP) for transient transfection.

Applications

  • Positive control for mRNA transfections
  • Optimization of mRNA transfection protocols

Detailed product information

Background information

StemMACS™ eGFP mRNA was designed for transient expression of the eGFP protein after transfection. Fluorescence of the eGFP protein can be easily detected by fluorescence microscopy or flow cytometry. StemMACS™ eGFP mRNA is therefore ideal for monitoring and optimizing transfection efficiency and is recommended as a positive control for mRNA transfections.

Detailed procedure

For satisfactory transfection results, use a transfection protocol optimized for your specific cell type. StemMACS™ eGFP mRNA or StemMACS™ Nuclear eGFP mRNA allow easy evaluation of transfection efficiency and are recommended as positive controls.

Quality description

StemMACS™ mRNAs are highly pure, in vitro-transcribed mRNAs that have been carefully optimized to ensure high level expression after transfection. Polyadenylation and enzymatic cap mimic a fully processed mature mRNA and ensure efficient translation. Incorporation of the modified nucleotides pseudouridine and 5-methylcytidine reduces the innate antiviral response to single-stranded mRNA. Robust expression of the encoded factor after transfection has been verified by immunofluorescence or flow cytometry.
StemMACS™ mRNAs are lyophilized from a 0.2 µM filtered solution for extended shelf-life.

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