StemMACS™ Cebpb mRNA encodes the transcription factor C/EBP-beta for transient transfection.

Data and images for StemMACS™ Cebpb mRNA, human

Figures

Figure 1

Cebpb expression after mRNA transfection:
Human BJ fibroblasts were transfected once with 150 ng StemMACS™ Cebpb mRNA and 75 ng StemMACS Nuclear eGFP mRNA. 24 hours post transfection, Cebpb expression (red) and colocalization with nuclear eGFP (green) were analyzed by immunofluorescence microscopy.
Transfection control
NeuroG2-transfected cells
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Figure 1

Cebpb expression after mRNA transfection:
Human BJ fibroblasts were transfected once with 150 ng StemMACS™ Cebpb mRNA and 75 ng StemMACS Nuclear eGFP mRNA. 24 hours post transfection, Cebpb expression (red) and colocalization with nuclear eGFP (green) were analyzed by immunofluorescence microscopy.
View details

Figure 1

Cebpb expression after mRNA transfection:
Human BJ fibroblasts were transfected once with 150 ng StemMACS™ Cebpb mRNA and 75 ng StemMACS Nuclear eGFP mRNA. 24 hours post transfection, Cebpb expression (red) and colocalization with nuclear eGFP (green) were analyzed by immunofluorescence microscopy.

Specifications for StemMACS™ Cebpb mRNA, human

Overview

StemMACS™ Cebpb mRNA encodes the transcription factor C/EBP-beta for transient transfection.

Detailed product information

Background information

CCAAT/enhancer-binding protein beta (C/EBP-beta) is a member of the basic leucine zipper (bZIP) containing family of transcription factors. It can function as a homodimer or in heterodimeric complexes with other C/EBP family members, such as C/EBPalpha, -beta or -gamma. C/EBP-beta plays a role in the regulation of genes that are important for immune and inflammatory responses. In addition, C/EBP-beta is transiently induced during the early stages of adipocyte differentiation and promotes adipogenesis in cooperation with other adipogenic factors such as PPAR-gamma. In pluripotent stem cell research overexpression of C/EBP-beta has been used to induce the differentiation into adipose tissue.

Detailed procedure

For satisfactory transfection results, use a transfection protocol optimized for your specific cell type. StemMACS™ eGFP mRNA or StemMACS™ Nuclear eGFP mRNA allow easy evaluation of transfection efficiency and are recommended as positive controls.

Quality description

StemMACS™ mRNAs are highly pure, in vitro-transcribed mRNAs that have been carefully optimized to ensure high level expression after transfection. Polyadenylation and enzymatic cap mimic a fully processed mature mRNA and ensure efficient translation. Incorporation of the modified nucleotides pseudouridine and 5-methylcytidine reduces the innate antiviral response to single-stranded mRNA. Robust expression of the encoded factor after transfection has been verified by immunofluorescence or flow cytometry.
StemMACS™ mRNAs are lyophilized from a 0.2 µM filtered solution for extended shelf-life.

References for StemMACS™ Cebpb mRNA, human

Publications

  1. Ahfeldt et al. (2012) Programming human pluripotent stem cells into white and brown adipocytes. Nat. Cell Biol. 14(2): 209-219

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StemMACS™ Cebpb mRNA, human

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