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Cre-mediated recombination in mouse ES cells: HM1 embryonic stem cells carrying a GFP reporter gene that is transcribed upon cre-mediated recombination were electroporated with 3 μg StemMACS™ Cre Recombinase mRNA and analyzed by flow cytometry 24 hours after transfection. 72% of all cells were GFP-positive indicating successful cre-mediated recombination. |
Untransfected cells | Transfected cells |
StemMACS™ Cre Recombinase mRNAFigure 1Cre-mediated recombination in mouse ES cells: HM1 embryonic stem cells carrying a GFP reporter gene that is transcribed upon cre-mediated recombination were electroporated with 3 μg StemMACS™ Cre Recombinase mRNA and analyzed by flow cytometry 24 hours after transfection. 72% of all cells were GFP-positive indicating successful cre-mediated recombination. | StemMACS™ Cre Recombinase mRNAFigure 1Cre-mediated recombination in mouse ES cells: HM1 embryonic stem cells carrying a GFP reporter gene that is transcribed upon cre-mediated recombination were electroporated with 3 μg StemMACS™ Cre Recombinase mRNA and analyzed by flow cytometry 24 hours after transfection. 72% of all cells were GFP-positive indicating successful cre-mediated recombination. |
Cre-mediated recombination in mouse ES cells: HM1 embryonic stem cells carrying a GFP reporter gene that is transcribed upon cre-mediated recombination were electroporated with 3 μg StemMACS™ Cre Recombinase mRNA and analyzed by flow cytometry 24 hours after transfection. 72% of all cells were GFP-positive indicating successful cre-mediated recombination. |
Untransfected cells | Transfected cells |
StemMACS™ Cre Recombinase mRNAFigure 1Cre-mediated recombination in mouse ES cells: HM1 embryonic stem cells carrying a GFP reporter gene that is transcribed upon cre-mediated recombination were electroporated with 3 μg StemMACS™ Cre Recombinase mRNA and analyzed by flow cytometry 24 hours after transfection. 72% of all cells were GFP-positive indicating successful cre-mediated recombination. | StemMACS™ Cre Recombinase mRNAFigure 1Cre-mediated recombination in mouse ES cells: HM1 embryonic stem cells carrying a GFP reporter gene that is transcribed upon cre-mediated recombination were electroporated with 3 μg StemMACS™ Cre Recombinase mRNA and analyzed by flow cytometry 24 hours after transfection. 72% of all cells were GFP-positive indicating successful cre-mediated recombination. |
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