The REAlease
®
CD4 (TIL) MicroBead Kit,human has been developed for positive selection of CD4
+
tumor-infiltrating leukocytes (TILs) from single-cell suspensions of solid human tumors as well as xenotransplanted tumors of mice with a humanized immune system. The kit contains an indirect magnetic labeling system for obtaining target cells free of MicroBeads and REAlease Biotin Complex.

Data and images for
REAlease
®
CD4 (TIL) MicroBead Kit
, human

Figures

Figure 1

Example of a separation using the REAlease® CD4 (TIL) MicroBead Kit.
A) A human ovarian carcinoma sample was dissociated using the gentleMACS™ Octo Dissociator with Heaters in combination with the Tumor Dissociation Kit, human (# 130-095-929). CD4
+
TILs were isolated from the single-cell suspension using the REAlease
®
CD4 (TIL) MicroBead Kit, LS Columns, and a MidiMACS™ Separator. Cells were fluorescently stained with CD4-VioGreen™ and analyzed by flow cytometry using the MACSQuant
®
Analyzer X. Cell debris and dead cells were excluded from the analysis based on scatter signals and 7-AAD fluorescence. B) The efficient removal of all labels was shown by using Anti-Biotin-APC to analyze the cells by flow cytometry for the presence of REAlease Biotin Complex. Directly after isolation, the cells showed staining of biotin ("MicroBead-free CD4
+
cells"), whereas the label-free CD4
+
cells after the REAlease Biotin Complex release were negative for biotin similar to the non-labeled cells before separation.
Release efficiency was higher than 99% for the REAlease Anti-Biotin MicroBeads (CD4 (TIL)).
A) Cell purity
Before separation
Label-free CD4
+
cells
View details

Figure 1

Example of a separation using the REAlease® CD4 (TIL) MicroBead Kit.
A) A human ovarian carcinoma sample was dissociated using the gentleMACS™ Octo Dissociator with Heaters in combination with the Tumor Dissociation Kit, human (# 130-095-929). CD4
+
TILs were isolated from the single-cell suspension using the REAlease
®
CD4 (TIL) MicroBead Kit, LS Columns, and a MidiMACS™ Separator. Cells were fluorescently stained with CD4-VioGreen™ and analyzed by flow cytometry using the MACSQuant
®
Analyzer X. Cell debris and dead cells were excluded from the analysis based on scatter signals and 7-AAD fluorescence. B) The efficient removal of all labels was shown by using Anti-Biotin-APC to analyze the cells by flow cytometry for the presence of REAlease Biotin Complex. Directly after isolation, the cells showed staining of biotin ("MicroBead-free CD4
+
cells"), whereas the label-free CD4
+
cells after the REAlease Biotin Complex release were negative for biotin similar to the non-labeled cells before separation.
Release efficiency was higher than 99% for the REAlease Anti-Biotin MicroBeads (CD4 (TIL)).
View details

Figure 1

Example of a separation using the REAlease® CD4 (TIL) MicroBead Kit.
A) A human ovarian carcinoma sample was dissociated using the gentleMACS™ Octo Dissociator with Heaters in combination with the Tumor Dissociation Kit, human (# 130-095-929). CD4
+
TILs were isolated from the single-cell suspension using the REAlease
®
CD4 (TIL) MicroBead Kit, LS Columns, and a MidiMACS™ Separator. Cells were fluorescently stained with CD4-VioGreen™ and analyzed by flow cytometry using the MACSQuant
®
Analyzer X. Cell debris and dead cells were excluded from the analysis based on scatter signals and 7-AAD fluorescence. B) The efficient removal of all labels was shown by using Anti-Biotin-APC to analyze the cells by flow cytometry for the presence of REAlease Biotin Complex. Directly after isolation, the cells showed staining of biotin ("MicroBead-free CD4
+
cells"), whereas the label-free CD4
+
cells after the REAlease Biotin Complex release were negative for biotin similar to the non-labeled cells before separation.
Release efficiency was higher than 99% for the REAlease Anti-Biotin MicroBeads (CD4 (TIL)).
B) Label-free cells: REAlease Biotin Complex release
Before separation
MicroBead-free CD4
+
cells
Label-free CD4
+
cells
View details

Figure 1

Example of a separation using the REAlease® CD4 (TIL) MicroBead Kit.
A) A human ovarian carcinoma sample was dissociated using the gentleMACS™ Octo Dissociator with Heaters in combination with the Tumor Dissociation Kit, human (# 130-095-929). CD4
+
TILs were isolated from the single-cell suspension using the REAlease
®
CD4 (TIL) MicroBead Kit, LS Columns, and a MidiMACS™ Separator. Cells were fluorescently stained with CD4-VioGreen™ and analyzed by flow cytometry using the MACSQuant
®
Analyzer X. Cell debris and dead cells were excluded from the analysis based on scatter signals and 7-AAD fluorescence. B) The efficient removal of all labels was shown by using Anti-Biotin-APC to analyze the cells by flow cytometry for the presence of REAlease Biotin Complex. Directly after isolation, the cells showed staining of biotin ("MicroBead-free CD4
+
cells"), whereas the label-free CD4
+
cells after the REAlease Biotin Complex release were negative for biotin similar to the non-labeled cells before separation.
Release efficiency was higher than 99% for the REAlease Anti-Biotin MicroBeads (CD4 (TIL)).
View details

Figure 1

Example of a separation using the REAlease® CD4 (TIL) MicroBead Kit.
A) A human ovarian carcinoma sample was dissociated using the gentleMACS™ Octo Dissociator with Heaters in combination with the Tumor Dissociation Kit, human (# 130-095-929). CD4
+
TILs were isolated from the single-cell suspension using the REAlease
®
CD4 (TIL) MicroBead Kit, LS Columns, and a MidiMACS™ Separator. Cells were fluorescently stained with CD4-VioGreen™ and analyzed by flow cytometry using the MACSQuant
®
Analyzer X. Cell debris and dead cells were excluded from the analysis based on scatter signals and 7-AAD fluorescence. B) The efficient removal of all labels was shown by using Anti-Biotin-APC to analyze the cells by flow cytometry for the presence of REAlease Biotin Complex. Directly after isolation, the cells showed staining of biotin ("MicroBead-free CD4
+
cells"), whereas the label-free CD4
+
cells after the REAlease Biotin Complex release were negative for biotin similar to the non-labeled cells before separation.
Release efficiency was higher than 99% for the REAlease Anti-Biotin MicroBeads (CD4 (TIL)).
View details

Figure 1

Example of a separation using the REAlease® CD4 (TIL) MicroBead Kit.
A) A human ovarian carcinoma sample was dissociated using the gentleMACS™ Octo Dissociator with Heaters in combination with the Tumor Dissociation Kit, human (# 130-095-929). CD4
+
TILs were isolated from the single-cell suspension using the REAlease
®
CD4 (TIL) MicroBead Kit, LS Columns, and a MidiMACS™ Separator. Cells were fluorescently stained with CD4-VioGreen™ and analyzed by flow cytometry using the MACSQuant
®
Analyzer X. Cell debris and dead cells were excluded from the analysis based on scatter signals and 7-AAD fluorescence. B) The efficient removal of all labels was shown by using Anti-Biotin-APC to analyze the cells by flow cytometry for the presence of REAlease Biotin Complex. Directly after isolation, the cells showed staining of biotin ("MicroBead-free CD4
+
cells"), whereas the label-free CD4
+
cells after the REAlease Biotin Complex release were negative for biotin similar to the non-labeled cells before separation.
Release efficiency was higher than 99% for the REAlease Anti-Biotin MicroBeads (CD4 (TIL)).

Specifications for
REAlease
®
CD4 (TIL) MicroBead Kit
, human

Overview

The REAlease
®
CD4 (TIL) MicroBead Kit,human has been developed for positive selection of CD4
+
tumor-infiltrating leukocytes (TILs) from single-cell suspensions of solid human tumors as well as xenotransplanted tumors of mice with a humanized immune system. The kit contains an indirect magnetic labeling system for obtaining target cells free of MicroBeads and REAlease Biotin Complex.

Detailed product information

Background information

The REAlease
®
CD4 (TIL) MicroBead Kit has been developed for the isolation of CD4
+
tumor-infiltrating leukocytes (TILs) from single-cell suspensions of solid human tumors as well as xenotransplanted tumors of mice with a humanized immune system. CD4 is expressed on T helper cells, at a lower level on monocytes and dendritic cells, and at a very low level on hematopoietic progenitor cells.
The REAlease CD4 (TIL) MicroBead Kit is an indirect magnetic labeling system that allows to obtain cells free of MicroBeads and the REAlease Biotin Complex.

Downstream applications

  • Isolation of specific CD4+ tumor-infiltrating T cell subsets. The selected CD4+ T helper cell population can be sorted according to a second marker of interest, for example, CD25, CD137 (4-1BB), CD223 (LAG-3), CD279 (PD1), or CD366 (TIM-3).
  • Isolation of CD4+ tumor-infiltrating T cells which need to be label-free.

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®
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, human

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