Clone:
REA338
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
ICFC
Alternative names:
POU5F1, OTF-3, OTF-4, Oct-3, Oct-4, Oct3/4A, Oct-3A, NF-A3

Specifications for Oct3/4 Isoform A Antibody, anti-human/mouse, REAfinity™

Overview

Clone REA338 recognizes the human octamer-binding transcription factor 3/4 isoform A (Oct3/4A) and the mouse Oct3/4. Oct3/4 is a homeodomain transcription factor belonging to the POU transcription factor family and is also known as POU domain class 5 transcription factor 1 (POU5F1). Oct3/4 is critical for the maintenance of totipotency in primate blastomeres and for pluripotency of the inner cell mass of developing mammalian embryos. This critical role of Oct3/4 is maintained in embryonic stem cells, the developing germline, and germline tumors, such as seminomas and embryonic teratocarcinomas. When inducing pluripotency in differentiated cells, Oct3/4 appears to be an essential, but not the only required transcription factor. Oct3/4 acts as a transcriptional activator of genes involved in maintenance of undifferentiated state and as a repressor of differentiation-specific genes.In human it encodes two chief isoforms that are generated by alternative splicing – Oct3/4A and Oct3/4b. Only the Oct3/4A protein consequently contains the unique N-terminal transactivation domain that regulates DNA binding of Oct3/4A to target promoters. It is only this full length Oct3/4A protein – that localizes to the nucleus, binds to Oct3/4-regulated promoters, and contributes to the maintenance of pluripotency and self-renewal.
Additional information: Clone REA338 displays negligible binding to Fc receptors.

Alternative names

POU5F1, OTF-3, OTF-4, Oct-3, Oct-4, Oct3/4A, Oct-3A, NF-A3

Detailed product information

Technical specifications

CloneREA338
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (I), human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman, mouse
AntigenOct3/4 Isoform A
Alternative names of antigenPOU5F1, OTF-3, OTF-4, Oct-3, Oct-4, Oct3/4A, Oct-3A, NF-A3
Molecular mass of antigen [kDa]39(refers to isoform A (OCT3A))
Distribution of antigenstem cells, embryonic germ cells, ES and iPS cells
Entrez Gene ID5460
RRIDAB_2784444, AB_2784445

Resources for Oct3/4 Isoform A Antibody, anti-human/mouse, REAfinity™

References for Oct3/4 Isoform A Antibody, anti-human/mouse, REAfinity™

Publications

  1. Nakagawa, E. et al. (2012) The novel function of Oct3/4 in mouse tooth development. Histochem. Cell Biol. 137(3): 367-376
  2. Niwa, H. et al. (2000) Quantitative expression of Oct-3/4 defines differentiation, dedifferentiation or self-renewal of ES cells. Nat. Genet. 24(4): 372-376
  3. Wey, E. et al. (1994) A human POU domain gene, mPOU, is expressed in developing brain and specific adult tissues. Eur. J. Biochem. 220(3): 753-762

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