Transcription factors are a set of protein molecules, that bind to a specific DNA sequence (promoter) in the gene and thereby control transcription of DNA to messenger RNA. Antibodies are commonly used for intracellular staining and subsequent identification and enumeration of specific transcription factors by flow cytometry. The Transcription Factor Staining Buffer Set has been developed for use in conjunction with antibodies targeting various transcription factors.

Data and images for Transcription Factor Staining Buffer Set

Figures

Figure 1

Following dot plots show intracellular staining with numerous antibodies using the Transcription Factor Staining Buffer Set for fixation and permeabilization of the cells. Staining with additional antibodies was performed as indicated in the plot. Cells were analyzed by flow cytometry using the MACSQuant
®
Analyzer. Cell debris were excluded from the analysis based on scatter signals.
View details

Figure 1

Following dot plots show intracellular staining with numerous antibodies using the Transcription Factor Staining Buffer Set for fixation and permeabilization of the cells. Staining with additional antibodies was performed as indicated in the plot. Cells were analyzed by flow cytometry using the MACSQuant
®
Analyzer. Cell debris were excluded from the analysis based on scatter signals.
View details

Figure 1

Following dot plots show intracellular staining with numerous antibodies using the Transcription Factor Staining Buffer Set for fixation and permeabilization of the cells. Staining with additional antibodies was performed as indicated in the plot. Cells were analyzed by flow cytometry using the MACSQuant
®
Analyzer. Cell debris were excluded from the analysis based on scatter signals.
View details

Figure 1

Following dot plots show intracellular staining with numerous antibodies using the Transcription Factor Staining Buffer Set for fixation and permeabilization of the cells. Staining with additional antibodies was performed as indicated in the plot. Cells were analyzed by flow cytometry using the MACSQuant
®
Analyzer. Cell debris were excluded from the analysis based on scatter signals.
View details

Figure 1

Following dot plots show intracellular staining with numerous antibodies using the Transcription Factor Staining Buffer Set for fixation and permeabilization of the cells. Staining with additional antibodies was performed as indicated in the plot. Cells were analyzed by flow cytometry using the MACSQuant
®
Analyzer. Cell debris were excluded from the analysis based on scatter signals.
View details

Figure 1

Following dot plots show intracellular staining with numerous antibodies using the Transcription Factor Staining Buffer Set for fixation and permeabilization of the cells. Staining with additional antibodies was performed as indicated in the plot. Cells were analyzed by flow cytometry using the MACSQuant
®
Analyzer. Cell debris were excluded from the analysis based on scatter signals.
View details

Figure 1

Following dot plots show intracellular staining with numerous antibodies using the Transcription Factor Staining Buffer Set for fixation and permeabilization of the cells. Staining with additional antibodies was performed as indicated in the plot. Cells were analyzed by flow cytometry using the MACSQuant
®
Analyzer. Cell debris were excluded from the analysis based on scatter signals.

Specifications for Transcription Factor Staining Buffer Set

Overview

Transcription factors are a set of protein molecules, that bind to a specific DNA sequence (promoter) in the gene and thereby control transcription of DNA to messenger RNA. Antibodies are commonly used for intracellular staining and subsequent identification and enumeration of specific transcription factors by flow cytometry. The Transcription Factor Staining Buffer Set has been developed for use in conjunction with antibodies targeting various transcription factors.