The Mouse IL-5 Secretion Assay was developed for the sensitive detection of murine IL-5-secreting cells.

Data and images for Mouse IL-5 Secretion Assay – Detection Kits

Figures

Figure 1

BALB/c mice were immunized and the spleen cells restimulated with KLH (keyhole limpet hemocyanin). The responding cells were stained according to secretion of IL-5 using the Mouse IL-5 Secretion Assay - Detection Kit.
In the stimulated sample, 0.085% of all CD4
+
T cells secreted IL-5. In the unstimulated control sample, only 0.007% of all CD4
+
T cells secreted IL-5.
Stimulated sample
Unstimulated control
View details

Figure 1

BALB/c mice were immunized and the spleen cells restimulated with KLH (keyhole limpet hemocyanin). The responding cells were stained according to secretion of IL-5 using the Mouse IL-5 Secretion Assay - Detection Kit.
In the stimulated sample, 0.085% of all CD4
+
T cells secreted IL-5. In the unstimulated control sample, only 0.007% of all CD4
+
T cells secreted IL-5.
View details

Figure 1

BALB/c mice were immunized and the spleen cells restimulated with KLH (keyhole limpet hemocyanin). The responding cells were stained according to secretion of IL-5 using the Mouse IL-5 Secretion Assay - Detection Kit.
In the stimulated sample, 0.085% of all CD4
+
T cells secreted IL-5. In the unstimulated control sample, only 0.007% of all CD4
+
T cells secreted IL-5.

Specifications for Mouse IL-5 Secretion Assay – Detection Kits

Overview

The Mouse IL-5 Secretion Assay was developed for the sensitive detection of murine IL-5-secreting cells.

Detailed product information

Background information

Interleukin-5 (IL-5) is predominantly secreted by CD4
+
T cells. It promotes growth and activation of eosinophils, is involved in a range of allergic reactions, and mediates immune reactions against parasites. IL-5 also acts on other cell types such as B cells.

Downstream applications

The Mouse IL-5 Secretion Assays allow studies on T
H
2 development and commitment. Furthermore, they can be used for two-color cytokine analysis or counterstaining of peptide-MHC tetramer PE-labeled cells.

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