CD4 Antibody, anti-mouse

Name: CD4 Antibody, anti-mouse
Availability: InStock

CD4 Antibody, anti-mouseExtended Validation

Clone:

GK1.5

Type of antibody:

Primary antibodies

Isotype:

rat IgG2bκ

Applications:

FC, MICS, IF, IHC

Alternative names:

L3T4, Ly-4, Leu-3, T4

Flow cytometry applications forCD4 Antibody, anti-mouse

APC
APC-Vio 770
Biotin
FITC
PE
PE-Vio 770
PerCP-Vio 700
VioBlue
VioGreen

Conjugate:

APC

Recommended dilution:

1:50

Remark:

The antibody is suited for staining of formaldehyde-fixed cells.

Tested:

QC tested

Products used:

130-116-523, 130-116-546

Protocols:

Cell surface flow cytometry staining protocol (PBS/EDTA/BSA 1:50)

Description:

Mouse splenocytes were stained with CD4 antibodies and analyzed by flow cytometry using the MACSQuant^® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence or 4',6-diamidino-2-phenylindole (DAPI) fluorescence, as in the case of tandem conjugates.

MICS (MACSima Imaging Cyclic Staining) applications forCD4 Antibody, anti-mouse

APC
FITC
PE

Conjugate:

APC

Recommended dilution:

1:50

Fixation:

Acetone

Tested:

during development

Species tested:

mouse

Products used:

130-116-523, 130-116-546

Compatible applications:

IF, IHC

Protocols:

Protocol for MICS experiments

Extended validation for CD4 Antibody, anti-mouse

Sensitivity

Performance comparison

Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.

Flow cytometric comparison of different clones for CD4. Splenocytes from BALB/c mice were stained with CD4 antibodies and with a suitable counterstaining. As a control, CD4 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Flow cytometric comparison of different clones for CD4. Splenocytes from BALB/c mice were stained with CD4 antibodies and with a suitable counterstaining. As a control, CD4 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Flow cytometric comparison of different clones for CD4. Splenocytes from BALB/c mice were stained with CD4 antibodies and with a suitable counterstaining. As a control, CD4 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Flow cytometric comparison of different clones for CD4. Splenocytes from BALB/c mice were stained with CD4 antibodies and with a suitable counterstaining. As a control, CD4 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Flow cytometric comparison of different clones for CD4. Splenocytes from BALB/c mice were stained with CD4 antibodies and with a suitable counterstaining. As a control, CD4 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Flow cytometric comparison of different clones for CD4. Splenocytes from BALB/c mice were stained with CD4 antibodies and with a suitable counterstaining. As a control, CD4 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Flow cytometric comparison of different clones for CD4. Splenocytes from BALB/c mice were stained with CD4 antibodies and with a suitable counterstaining. As a control, CD4 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Fixation data

To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.

No fixation

Post fixation

Comparison of staining pattern on non-fixed and fixed cells using CD4 (GK1.5). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Comparison of staining pattern on non-fixed and fixed cells using CD4 (GK1.5). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD4 Antibody, anti-mouse

Overview

Clone GK1.5 recognizes the mouse CD4 (L3T4) antigen. In mice, CD4 is expressed on T helper cells, regulatory T cells, and at lower levels on subpopulations of NKT cells and dendritic cells. It is furthermore detected on most thymocytes (CD4

⁺

CD8

⁺

and CD4

⁺

CD8

^–

thymocytes).

Alternative names

L3T4, Ly-4, Leu-3, T4

Detailed product information

Technical specifications

Clone	GK1.5
Clonality	monoclonal
Isotype	rat IgG2bκ
Isotype control	Isotype Control Antibody, rat IgG2b
Host	rat
Type of antibody	Primary antibodies
Species	mouse
Antigen	CD4
Alternative names of antigen	L3T4, Ly-4, Leu-3, T4
Molecular mass of antigen [kDa]	48
Distribution of antigen	dendritic cells, granulocytes, Langerhans cells, macrophages, monocytes, T cells, lymphocytes, neutrophils, T helper cells, thymocytes
Entrez Gene ID	12504
RRID	AB_2727604, AB_2751634, AB_2751588, AB_2752205, AB_2752185, AB_2752219, AB_2819404, AB_2819535, AB_2819537, AB_2819676, AB_2659916, AB_2659917, AB_2727589

Resources for CD4 Antibody, anti-mouse

Documents and Protocols

Scientific posters

Analysis of T cells made easy – complete workflow improves the isolation and analysis of T cells

Certificates

Please follow this

link

to search for Certificates of Analysis (CoA) by lot number.

Applications

Extended validation