Mouse IL-4 Secretion Assay – Cell Enrichment and Detection Kit (PE)

Name: Mouse IL-4 Secretion Assay – Cell Enrichment and Detection Kit (PE)
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Mouse IL-4 Secretion Assay – Cell Enrichment and Detection Kit (PE)

The Mouse IL-4 Secretion Assay -Cell Enrichment and Detection Kit was developed for the sensitive detection as well as the enrichment of murine IL-4-secreting cells.

Product data and images for Mouse IL-4 Secretion Assay – Cell Enrichment and Detection Kit (PE)

Figures

Figure 1

BALB/c mice were immunized and the spleen cells restimulated with HEL as described for the Mouse IFN-γ Secretion Assay. The responding cells were stained and enriched according to secretion of IL-4 using the Mouse IL-4 Secretion Assay - Cell Enrichment and Detection Kit.

In the stimulated sample, 236 IL-4-secreting CD4

⁺

T cells were enriched per 10

⁶

CD4

⁺

T cells using MS Columns and a MiniMACS™ Separator. In the unstimulated control sample, only 48 IL-4-secreting CD4

⁺

T cells were enriched per 10

⁶

CD4

⁺

T cells.

Stimulated sample

Before enrichment	After enrichment
View details Mouse IL-4 Secretion Assay – Cell Enrichment and Detection Kit (PE) Figure 1 BALB/c mice were immunized and the spleen cells restimulated with HEL as described for the Mouse IFN-γ Secretion Assay. The responding cells were stained and enriched according to secretion of IL-4 using the Mouse IL-4 Secretion Assay - Cell Enrichment and Detection Kit. In the stimulated sample, 236 IL-4-secreting CD4 ⁺ T cells were enriched per 10 ⁶ CD4 ⁺ T cells using MS Columns and a MiniMACS™ Separator. In the unstimulated control sample, only 48 IL-4-secreting CD4 ⁺ T cells were enriched per 10 ⁶ CD4 ⁺ T cells.	View details Mouse IL-4 Secretion Assay – Cell Enrichment and Detection Kit (PE) Figure 1 BALB/c mice were immunized and the spleen cells restimulated with HEL as described for the Mouse IFN-γ Secretion Assay. The responding cells were stained and enriched according to secretion of IL-4 using the Mouse IL-4 Secretion Assay - Cell Enrichment and Detection Kit. In the stimulated sample, 236 IL-4-secreting CD4 ⁺ T cells were enriched per 10 ⁶ CD4 ⁺ T cells using MS Columns and a MiniMACS™ Separator. In the unstimulated control sample, only 48 IL-4-secreting CD4 ⁺ T cells were enriched per 10 ⁶ CD4 ⁺ T cells.
Unstimulated control

Before enrichment	After enrichment
View details Mouse IL-4 Secretion Assay – Cell Enrichment and Detection Kit (PE) Figure 1 BALB/c mice were immunized and the spleen cells restimulated with HEL as described for the Mouse IFN-γ Secretion Assay. The responding cells were stained and enriched according to secretion of IL-4 using the Mouse IL-4 Secretion Assay - Cell Enrichment and Detection Kit. In the stimulated sample, 236 IL-4-secreting CD4 ⁺ T cells were enriched per 10 ⁶ CD4 ⁺ T cells using MS Columns and a MiniMACS™ Separator. In the unstimulated control sample, only 48 IL-4-secreting CD4 ⁺ T cells were enriched per 10 ⁶ CD4 ⁺ T cells.	View details Mouse IL-4 Secretion Assay – Cell Enrichment and Detection Kit (PE) Figure 1 BALB/c mice were immunized and the spleen cells restimulated with HEL as described for the Mouse IFN-γ Secretion Assay. The responding cells were stained and enriched according to secretion of IL-4 using the Mouse IL-4 Secretion Assay - Cell Enrichment and Detection Kit. In the stimulated sample, 236 IL-4-secreting CD4 ⁺ T cells were enriched per 10 ⁶ CD4 ⁺ T cells using MS Columns and a MiniMACS™ Separator. In the unstimulated control sample, only 48 IL-4-secreting CD4 ⁺ T cells were enriched per 10 ⁶ CD4 ⁺ T cells.
* Percentage represents frequency among CD4⁺ T cells.

Specifications for Mouse IL-4 Secretion Assay – Cell Enrichment and Detection Kit (PE)

Overview

The Mouse IL-4 Secretion Assay -Cell Enrichment and Detection Kit was developed for the sensitive detection as well as the enrichment of murine IL-4-secreting cells.

Detailed product information

Background information

Interleukin-4 (IL-4) is predominantly secreted by activated CD4

⁺

memory and effector T

_H

2 cells, basophils, and mast cells. It induces and supports humoral immune responses for the neutralization of extracellular pathogens. The T2 types of immune mechanisms can also be involved in immunologic disorders such as IgE-mediated allergy.

Downstream applications

The assays have been used for research on T

_H

2 development and commitment

^1,3

and to investigate the regulation of IL-4 expression

^2,4

. The Mouse IL-4 Secretion Assay was also used for isolation and analysis of IL-4-secreting NKT cells after stimulation with glycolipids.

⁵

Columns

MS, LS, or autoMACS

^®

Columns.

References for Mouse IL-4 Secretion Assay – Cell Enrichment and Detection Kit (PE)

Publications

Ouyang, W. et al. (2000) Stat6-independent GATA-3 autoactivation directs IL-4-independent Th2 development and commitment. Immunity 12: 27-37
Hu-Li, J. et al. (2001) Regulation of expression of IL-4 alleles: analysis using a chimeric GFP/IL-4 gene. Immunity 14: 1-11
Löhning, M. et al. (2003) Establishment of memory for IL-10 expression in developing T helper 2 cells requires repetitive IL-4 costimulation and does not impair proliferation. Proc. Natl. Acad. Sci. U.S.A. 100: 12307-12312
Guo, L. et al. (2004) Probabilistic regulation of IL-4 production in Tʜ2 cells: accessibility at the Il4 locus. Immunity 20: 193-203
Yang, J. Q. et al. (2003) Immunoregulatory role of CD1d in the hydrocarbon oil-induced model of lupus nephritis. J. Immunol. 171: 2142-2153

Data and images