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BALB/c mice were immunized and the spleen cells restimulated with HEL as described for the Mouse IFN-γ Secretion Assay. The responding cells were stained and enriched according to secretion of IL-4 using the Mouse IL-4 Secretion Assay - Cell Enrichment and Detection Kit. In the stimulated sample, 236 IL-4-secreting CD4 + T cells were enriched per 10 6 CD4 + T cells using MS Columns and a MiniMACS™ Separator. In the unstimulated control sample, only 48 IL-4-secreting CD4 + T cells were enriched per 10 6 CD4 + T cells. |
Stimulated sample |
Before enrichment | After enrichment |
Mouse IL-4 Secretion Assay – Cell Enrichment and Detection Kit (PE)Figure 1BALB/c mice were immunized and the spleen cells restimulated with HEL as described for the Mouse IFN-γ Secretion Assay. The responding cells were stained and enriched according to secretion of IL-4 using the Mouse IL-4 Secretion Assay - Cell Enrichment and Detection Kit. In the stimulated sample, 236 IL-4-secreting CD4 + T cells were enriched per 10 6 CD4 + T cells using MS Columns and a MiniMACS™ Separator. In the unstimulated control sample, only 48 IL-4-secreting CD4 + T cells were enriched per 10 6 CD4 + T cells. | Mouse IL-4 Secretion Assay – Cell Enrichment and Detection Kit (PE)Figure 1BALB/c mice were immunized and the spleen cells restimulated with HEL as described for the Mouse IFN-γ Secretion Assay. The responding cells were stained and enriched according to secretion of IL-4 using the Mouse IL-4 Secretion Assay - Cell Enrichment and Detection Kit. In the stimulated sample, 236 IL-4-secreting CD4 + T cells were enriched per 10 6 CD4 + T cells using MS Columns and a MiniMACS™ Separator. In the unstimulated control sample, only 48 IL-4-secreting CD4 + T cells were enriched per 10 6 CD4 + T cells. |
Unstimulated control |
Before enrichment | After enrichment |
Mouse IL-4 Secretion Assay – Cell Enrichment and Detection Kit (PE)Figure 1BALB/c mice were immunized and the spleen cells restimulated with HEL as described for the Mouse IFN-γ Secretion Assay. The responding cells were stained and enriched according to secretion of IL-4 using the Mouse IL-4 Secretion Assay - Cell Enrichment and Detection Kit. In the stimulated sample, 236 IL-4-secreting CD4 + T cells were enriched per 10 6 CD4 + T cells using MS Columns and a MiniMACS™ Separator. In the unstimulated control sample, only 48 IL-4-secreting CD4 + T cells were enriched per 10 6 CD4 + T cells. | Mouse IL-4 Secretion Assay – Cell Enrichment and Detection Kit (PE)Figure 1BALB/c mice were immunized and the spleen cells restimulated with HEL as described for the Mouse IFN-γ Secretion Assay. The responding cells were stained and enriched according to secretion of IL-4 using the Mouse IL-4 Secretion Assay - Cell Enrichment and Detection Kit. In the stimulated sample, 236 IL-4-secreting CD4 + T cells were enriched per 10 6 CD4 + T cells using MS Columns and a MiniMACS™ Separator. In the unstimulated control sample, only 48 IL-4-secreting CD4 + T cells were enriched per 10 6 CD4 + T cells. |
* Percentage represents frequency among CD4+ T cells. |
** Percentage represents frequency among enriched cells. |
BALB/c mice were immunized and the spleen cells restimulated with HEL as described for the Mouse IFN-γ Secretion Assay. The responding cells were stained and enriched according to secretion of IL-4 using the Mouse IL-4 Secretion Assay - Cell Enrichment and Detection Kit. In the stimulated sample, 236 IL-4-secreting CD4 + T cells were enriched per 10 6 CD4 + T cells using MS Columns and a MiniMACS™ Separator. In the unstimulated control sample, only 48 IL-4-secreting CD4 + T cells were enriched per 10 6 CD4 + T cells. |
Stimulated sample |
Before enrichment | After enrichment |
Mouse IL-4 Secretion Assay – Cell Enrichment and Detection Kit (PE)Figure 1BALB/c mice were immunized and the spleen cells restimulated with HEL as described for the Mouse IFN-γ Secretion Assay. The responding cells were stained and enriched according to secretion of IL-4 using the Mouse IL-4 Secretion Assay - Cell Enrichment and Detection Kit. In the stimulated sample, 236 IL-4-secreting CD4 + T cells were enriched per 10 6 CD4 + T cells using MS Columns and a MiniMACS™ Separator. In the unstimulated control sample, only 48 IL-4-secreting CD4 + T cells were enriched per 10 6 CD4 + T cells. | Mouse IL-4 Secretion Assay – Cell Enrichment and Detection Kit (PE)Figure 1BALB/c mice were immunized and the spleen cells restimulated with HEL as described for the Mouse IFN-γ Secretion Assay. The responding cells were stained and enriched according to secretion of IL-4 using the Mouse IL-4 Secretion Assay - Cell Enrichment and Detection Kit. In the stimulated sample, 236 IL-4-secreting CD4 + T cells were enriched per 10 6 CD4 + T cells using MS Columns and a MiniMACS™ Separator. In the unstimulated control sample, only 48 IL-4-secreting CD4 + T cells were enriched per 10 6 CD4 + T cells. |
Unstimulated control |
Before enrichment | After enrichment |
Mouse IL-4 Secretion Assay – Cell Enrichment and Detection Kit (PE)Figure 1BALB/c mice were immunized and the spleen cells restimulated with HEL as described for the Mouse IFN-γ Secretion Assay. The responding cells were stained and enriched according to secretion of IL-4 using the Mouse IL-4 Secretion Assay - Cell Enrichment and Detection Kit. In the stimulated sample, 236 IL-4-secreting CD4 + T cells were enriched per 10 6 CD4 + T cells using MS Columns and a MiniMACS™ Separator. In the unstimulated control sample, only 48 IL-4-secreting CD4 + T cells were enriched per 10 6 CD4 + T cells. | Mouse IL-4 Secretion Assay – Cell Enrichment and Detection Kit (PE)Figure 1BALB/c mice were immunized and the spleen cells restimulated with HEL as described for the Mouse IFN-γ Secretion Assay. The responding cells were stained and enriched according to secretion of IL-4 using the Mouse IL-4 Secretion Assay - Cell Enrichment and Detection Kit. In the stimulated sample, 236 IL-4-secreting CD4 + T cells were enriched per 10 6 CD4 + T cells using MS Columns and a MiniMACS™ Separator. In the unstimulated control sample, only 48 IL-4-secreting CD4 + T cells were enriched per 10 6 CD4 + T cells. |
* Percentage represents frequency among CD4+ T cells. |
** Percentage represents frequency among enriched cells. |
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