Granulocyte-macrophage colony–stimulating factor (GM-CSF) is a hematopoietic growth factor, which is essential for proliferation and development of granulocyte and monocyte/macrophage progenitors.
MACS GMP Recombinant Human GM-CSF is designed for
ex vivo
cell culture processing. No animal- or human-derived materials were used for the manufacture of this product, unless otherwise stated in the respective Certificate of Origin. The product is lyophilized without carrier protein or preservatives.

Data and images for
MACS
®
GMP Recombinant Human GM-CSF

Figures

Figure 1

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Mass spectrometry analysis (ESI-MS) of MACS GMP Recombinant Human GM-CSF. The peak corresponds to the calculated molecular mass of 14474 Da.

Figure 1

Mass spectrometry analysis (ESI-MS) of MACS GMP Recombinant Human GM-CSF. The peak corresponds to the calculated molecular mass of 14474 Da.

Figure 2

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MACS GMP Recombinant Human GM-CSF biological activity.
Activity of Human GM-CSF, GMP grade, (blue bar) was compared to other commercially available products (black and grey bar).

Figure 2

MACS GMP Recombinant Human GM-CSF biological activity.
Activity of Human GM-CSF, GMP grade, (blue bar) was compared to other commercially available products (black and grey bar).

Figure 3

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MACS GMP Recombinant Human GM-CSF batch-to-batch variability.
Activity of three different production batches of Human MACS GMP GM-CSF.

Figure 3

MACS GMP Recombinant Human GM-CSF batch-to-batch variability.
Activity of three different production batches of Human MACS GMP GM-CSF.

Specifications for
MACS
®
GMP Recombinant Human GM-CSF

Overview

Granulocyte-macrophage colony–stimulating factor (GM-CSF) is a hematopoietic growth factor, which is essential for proliferation and development of granulocyte and monocyte/macrophage progenitors.
MACS GMP Recombinant Human GM-CSF is designed for
ex vivo
cell culture processing. No animal- or human-derived materials were used for the manufacture of this product, unless otherwise stated in the respective Certificate of Origin. The product is lyophilized without carrier protein or preservatives.

Detailed product information

Background information

Beside its role as growth factor for granulocyte and monocyte precursors, GM-CSF also functions as a growth factor for erythroid and megakaryocytic precursor cells in conjunction with erythropoietin. GM-CSF is secreted by various cell types including T cells, macrophages, endothelial cells, and fibroblasts in response to inflammatory stimuli and cytokines. In addition, GM-CSF is a potent chemoattractant for neutrophils and eosinophils and enhances the effector functions of neutrophils and macrophages.

Applications

MACS GMP Recombinant Human GM-CSF can be used for a variety of applications, including the
ex vivo
generation of human dendritic cells from enriched CD14
+
monocytes.
2–4

Biological activity

  • The specific activity is determined by proliferation assay according to Kitamura
    et al.1
    using TF-1 cells provided by the German Resource Centre for Biological Material (DSMZ). The proliferation assay was calibrated with the international standard for human GM-CSF (NIBSC code 88/646) provided by the National Institute for Biological Standards and Control.
  • Specific activity: ≥ 5×
    10
    6
    IU/mg

Quality statement

MACS GMP Products are manufactured and tested under a quality management system (ISO 13485) and are in compliance with relevant GMP guidelines. They are designed following the recommendations of USP <1043> on ancillary materials.

Disclaimer

MACS GMP Products are for research use and
ex vivo
cell culture processing only, and are not intended for human
in vivo
applications. For regulatory status in the USA, please contact your local representative.

Resources for
MACS
®
GMP Recombinant Human GM-CSF

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for
MACS
®
GMP Recombinant Human GM-CSF

Publications

  1. Kitamura, T. et al. (1989) Establishment and characterization of a unique human cell line that proliferates dependently on GM-CSF, IL-3, or erythropoietin. J. Cell. Physiol. 140: 323-334
  2. Bender, A. et al. (1996) Improved methods for the generation of dendritic cells from nonproliferating progenitors in human blood. J. Immunol. Methods 196: 121-135
  3. Romani, N. et al. (1996) Generation of mature dendritic cells from human blood. An improved method with special regard to clinical applicability. J. Immunol. Methods 196: 137-151
  4. Jonuleit et al. (1997) Pro-inflammatory cytokines and prostaglandins induce maturation of potent immunostimulatory dendritic cells under fetal calf serum-free conditions. Eur. J. Immunol. 27: 3135-3142

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®
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