Alternative names:
CSF2

Data and images for Human GM-CSF

Figures

Figure 1

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Human GM-CSF activity assay.
The biological activity of Human GM-CSF, premium grade was determined by proliferation assay using TF-1 cells. Activity of Human GM-CSF, premium grade, (red line) was compared to another commercially available product (black line).

Figure 1

Human GM-CSF activity assay.
The biological activity of Human GM-CSF, premium grade was determined by proliferation assay using TF-1 cells. Activity of Human GM-CSF, premium grade, (red line) was compared to another commercially available product (black line).

Figure 2

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SDS-PAGE of Human GM-CSF, premium grade
under reduced (R) and non-reduced (NR) conditions.

Figure 2

SDS-PAGE of Human GM-CSF, premium grade
under reduced (R) and non-reduced (NR) conditions.

Figure 3

View details
Human GM-CSF biological activity.
Activity of Human GM-CSF, premium grade (red bar) was compared to another commercially available product (black bar).

Figure 3

Human GM-CSF biological activity.
Activity of Human GM-CSF, premium grade (red bar) was compared to another commercially available product (black bar).

Figure 4

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Mass spectrometry analysis (ESI-MS) of Human GM-CSF, premium grade. The peak corresponds to the calculated molecular mass of 14474 Da.

Figure 4

Mass spectrometry analysis (ESI-MS) of Human GM-CSF, premium grade. The peak corresponds to the calculated molecular mass of 14474 Da.

Specifications for Human GM-CSF

Overview

Recombinant human GM-CSF induces the differentiation of granulocytes, monocytes, and macrophages. The hematopoietic cytokine is a crucial part of the immune/inflammatory path and serves as both a survival and activation signal for mature myeloid cells. The recombinant granulocyte-macrophage colony-stimulating factor (GM-CSF) has been developed for use in cell culture, differentiation studies, and functional assays.

Applications

Human GM-CSF can be used for a variety of applications including:
  • Cultivation of hematopoietic progenitor cells from human bone marrow in semi-solid medium.
  • In vitro generation of Mo-DCs together with Human IL-4.
  • In vitro differentiation of CD34+ cells towards eosinophils.
  • Migration assays for eosinophils.

Alternative names

CSF2

Detailed product information

Background information

GM-CSF is a hematopoietic growth factor, which is essential for proliferation and development of granulocyte and monocyte/macrophage progenitors. It also functions as a growth factor for erythroid and megakaryocytic precursor cells in conjunction with erythropoietin. GM-CSF is secreted by various cell types including T cells, macrophages, endothelial cells, and fibroblasts in response to inflammatory stimuli and cytokines. In addition, GM-CSF is a potent chemoattractant for neutrophils and eosinophils and enhances the effector functions of neutrophils and macrophages.

Biological activity

  • Proliferation of TF-1 cells (NIBSC 88/646)
  • research grade: ≥ 2×
    10
    6
    IU/mg
  • premium grade: ≥ 5×
    10
    6
    IU/mg
    (Typical specific activity: ≥ 1.2×
    10
    7
    IU/mg
    )
  • We measure the biological activity of each batch of MACS Premium-Grade Cytokines and state the results in the Certificate of Analysis (CoA). Based on the lot-specific activity exact doses of active cytokine can be applied to cell culture experiments. This allows for reproducible cell culture conditions without the need for time-consuming lot-to-lot testing.

Quality description

Research-grade
cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.
Premium-grade
cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/μg (<0.1 EU/μg), and purities are >97%. Lot-specific activities are stated in the Certificate of Analysis (www. miltenyibiotec.com/certificates).

Resources for Human GM-CSF

Documents and Protocols

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

Reviews for Human GM-CSF

Differentiation of M1 Macrophages from Human PBMCs / Monocytes

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Human GM-CSF, premium grade (130-093-864)

Here, our aim was to to differentiate M1 Macrophages from human PBMCs after extracting monocytes with the Pan Monocyte Isolation Kit II, human using different concentrations of GM-CSF.

Differentiation of M1 Macrophages from Human PBMCs / Monocytes

  • 1
  • 2
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  • 5

Human GM-CSF, premium grade (130-093-865)

Here, our aim was to to differentiate M1 Macrophages from human PBMCs after extracting monocytes with the Pan Monocyte Isolation Kit II, human using different concentrations of GM-CSF.

Differentiation of M1 Macrophages from Human PBMCs / Monocytes

  • 1
  • 2
  • 3
  • 4
  • 5

Human GM-CSF, premium grade (130-093-866)

Here, our aim was to to differentiate M1 Macrophages from human PBMCs after extracting monocytes with the Pan Monocyte Isolation Kit II, human using different concentrations of GM-CSF.

Differentiation of M1 Macrophages from Human PBMCs / Monocytes

  • 1
  • 2
  • 3
  • 4
  • 5

Human GM-CSF, premium grade (130-093-867)

Here, our aim was to to differentiate M1 Macrophages from human PBMCs after extracting monocytes with the Pan Monocyte Isolation Kit II, human using different concentrations of GM-CSF.

Differentiation of M1 Macrophages from Human PBMCs / Monocytes

  • 1
  • 2
  • 3
  • 4
  • 5

Human GM-CSF, premium grade (130-093-868)

Here, our aim was to to differentiate M1 Macrophages from human PBMCs after extracting monocytes with the Pan Monocyte Isolation Kit II, human using different concentrations of GM-CSF.

References for Human GM-CSF

Publications

  1. Kitamura, T. et al. (1989) Establishment and characterization of a unique human cell line that proliferates dependently on GM-CSF, IL-3, or erythropoietin. J. Cell. Physiol. 140: 323-334
  2. Ulfman, L. H. et al. (2008)
    Homeostatic intracellular-free Ca
    2+
    is permissive for Pap1-mediated constitutive activation of α
    4
    integrins on eosinophils.
    J. Immunol. 180: 5512-5519
  3. Bacher, P. et al. (2014) Antigen-specific expansion of human regulatory T cells as a major tolerance mechanism against mucosal fungi. Mucosal Immunol 7(4): 916-928
  4. Brault, J. et al. (2014)
    Optimized generation of functional neutrophils and macrophages from patient-specific induced pluripotent stem cells:
    ex vivo
    models of X
    0
    -linked, AR22
    0
    - and AR47
    0
    - chronic granulomatous diseases.
    Biores Open Access 3(6): 311-326
  5. Chase A. J. et al. (2011)
    Impairment of CD4
    +
    T cell polarization by dengue virus-infected dendritic cells.
    J. Infect. Dis. 203: 1763-1774
  6. Dighe N. et al. (2014) Long-term reproducible expression in human fetal liver hematopoietic stem cells with a UCOE-based lentiviral vector. PLoS One 9(8): e104805
  7. Kaebisch R. et al. (2014) Helicobacter pylori cytotoxin-associated gene A impairs human dendritic cell maturation and function through IL-10-mediated activation of STAT3. J. Immunol. 192: 316-323
  8. Koning F. A. et al. (2009) Defining APOBEC3 expression patterns in human tissues and hematopoietic cell subsets. J. Virol. 83: 9474-9485
  9. Laurenti, E. et al. (2015) CDK6 levels regulate quiescence exit in human hematopoietic stem cells. Cell Stem Cell 16(3): 302-313
  10. Guery L. et al. (2014) Fine-tuning nucleophosmin in macrophage differentiation and activation. Blood 118: 4694-4704

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