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As a global market leader with numerous subsidiaries and distributors, Miltenyi Biotec is committed to providing our customers around the world with the highest quality products. In addition to direct selling in more than 20 countries in North America, Europe and Asia/Pacific, Miltenyi Biotec also provides support for our customers through an extensive distributor network covering dozens of additional countries.
The MACSprep™ PBMC Isolation Kit has been developed for the fast isolation of human peripheral blood mononuclear cells (PBMCs) from 2–10 mL of freshly drawn anticoagulated whole blood without density gradient centrifugation. Isolated PBMCs are suitable for various downstream applications, such as co-cultures like functional immune cell assays, cytokine expression studies, or immune monitoring.
Untouched isolation of PBMCs from human whole blood
Untouched PBMCs from high viability and purity can be isolated from human whole blood using the MACSprep™ PBMC Isolation Kit, a MACSmix™ Tube Rotator, and a LS Column.
Before separation
After separation
Untouched PBMCs were isolated from 10 mL of human EDTA-anticoagulated whole blood using the MACSprep™ PBMC Isolation Kit, a MACSmix™ Tube Rotator, and an LS Column. The isolated cells were fluorescently stained with CD45-VioBlue® , CD3-FITC, CD4-VioGreen™, CD8-APC-Vio® 770, CD14-APC, CD16-PE, CD56-PE, CD20-PE-Vio770, and 7-AAD and analyzed by flow cytometry using the MACSQuant® Analyzer. Cell debris, non-leukocytes, and dead cells were excluded from the analysis based on CD45 expression, scatter signals, and 7-AAD.
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