Name: IL-17 Secretion Assay – Cell Enrichment and Detection Kit (PE), human
Availability: InStock

Product data and images for IL-17 Secretion Assay – Cell Enrichment and Detection Kit (PE), human

Figures

Figure 1

PBMCs were stimulated with CytoStim for 4 hours or left untreated. Both samples were treated with a CD40 antibody to allow the surface detection of CD154. Cells were enriched by using the IL-17 Secretion Assay – Cell Enrichment and Detection Kit. The dot plots show stimulated and unstimulated cells before and after separation. Secretion of IL-17 by CD154

⁺

cells is demonstrated. Data were gated on viable CD4

⁺

cells.

Restimulated sample

Before enrichment	After enrichment
View details Figure 1 PBMCs were stimulated with CytoStim for 4 hours or left untreated. Both samples were treated with a CD40 antibody to allow the surface detection of CD154. Cells were enriched by using the IL-17 Secretion Assay – Cell Enrichment and Detection Kit. The dot plots show stimulated and unstimulated cells before and after separation. Secretion of IL-17 by CD154 ⁺ cells is demonstrated. Data were gated on viable CD4 ⁺ cells.	View details Figure 1 PBMCs were stimulated with CytoStim for 4 hours or left untreated. Both samples were treated with a CD40 antibody to allow the surface detection of CD154. Cells were enriched by using the IL-17 Secretion Assay – Cell Enrichment and Detection Kit. The dot plots show stimulated and unstimulated cells before and after separation. Secretion of IL-17 by CD154 ⁺ cells is demonstrated. Data were gated on viable CD4 ⁺ cells.
Without restimulation

Before enrichment

After enrichment

View details

Figure 1

⁺

cells is demonstrated. Data were gated on viable CD4

⁺

cells.

View details

Figure 1

⁺

cells is demonstrated. Data were gated on viable CD4

⁺

cells.

Specifications for IL-17 Secretion Assay – Cell Enrichment and Detection Kit (PE), human

Overview

The IL-17 Secretion Assay – Cell Enrichment and Detection Kit was developed for the sensitive detection and enrichment of viable human IL-17–secreting leukocytes from PBMCs.

The used Anti-PE MicroBeads UltraPure enhances sensitivity and minimizes background of debris and dead cells.

Detailed product information

Background information

Interleukin 17 (IL-17) is produced by CD4

⁺

T helper cells, a T cell subset termed Th17, which also secrete IL-17F and IL-22 and express the NK cell marker CD161

. IL-17 secretion has also been described for other cell types, such as CD8

⁺

memory T cells.

Th17 cells are involved in the recruitment of neutrophils to control early stages of infections by a number of pathogens, such as extracellular bacteria and fungi. IL-17 and Th17 cells play an important role in immune-mediated inflammatory diseases, such as rheumatoid arthritis, psoriasis, multiple sclerosis, asthma, and inflammatory bowel disease.

Columns

MS, LS, or autoMACS

^®

Columns.

References for IL-17 Secretion Assay – Cell Enrichment and Detection Kit (PE), human

Publications

Cosmi, L. et al. (2008)
Human interleukin 17–producing cells originate from a CD161
⁺
CD4
⁺
T cell precursor.
J. Exp. Med. 205: 1903-1916

Data and images

Data and images for IL-17 Secretion Assay – Cell Enrichment and Detection Kit (PE), human

Figures

Figure 1

⁺

cells is demonstrated. Data were gated on viable CD4

⁺

cells.

Restimulated sample

Before enrichment	After enrichment
View details Figure 1 PBMCs were stimulated with CytoStim for 4 hours or left untreated. Both samples were treated with a CD40 antibody to allow the surface detection of CD154. Cells were enriched by using the IL-17 Secretion Assay – Cell Enrichment and Detection Kit. The dot plots show stimulated and unstimulated cells before and after separation. Secretion of IL-17 by CD154 ⁺ cells is demonstrated. Data were gated on viable CD4 ⁺ cells.	View details Figure 1 PBMCs were stimulated with CytoStim for 4 hours or left untreated. Both samples were treated with a CD40 antibody to allow the surface detection of CD154. Cells were enriched by using the IL-17 Secretion Assay – Cell Enrichment and Detection Kit. The dot plots show stimulated and unstimulated cells before and after separation. Secretion of IL-17 by CD154 ⁺ cells is demonstrated. Data were gated on viable CD4 ⁺ cells.
Without restimulation

Before enrichment

After enrichment

View details

Figure 1

⁺

cells is demonstrated. Data were gated on viable CD4

⁺

cells.

View details

Figure 1

⁺

cells is demonstrated. Data were gated on viable CD4

⁺

cells.

Specifications

Specifications for IL-17 Secretion Assay – Cell Enrichment and Detection Kit (PE), human

Overview

The IL-17 Secretion Assay – Cell Enrichment and Detection Kit was developed for the sensitive detection and enrichment of viable human IL-17–secreting leukocytes from PBMCs.

The used Anti-PE MicroBeads UltraPure enhances sensitivity and minimizes background of debris and dead cells.

Detailed product information

Background information

Interleukin 17 (IL-17) is produced by CD4

⁺

T helper cells, a T cell subset termed Th17, which also secrete IL-17F and IL-22 and express the NK cell marker CD161

. IL-17 secretion has also been described for other cell types, such as CD8

⁺

memory T cells.

Columns

MS, LS, or autoMACS

^®

Columns.

References

References for IL-17 Secretion Assay – Cell Enrichment and Detection Kit (PE), human

Publications

Cosmi, L. et al. (2008)
Human interleukin 17–producing cells originate from a CD161
⁺
CD4
⁺
T cell precursor.
J. Exp. Med. 205: 1903-1916

Productfinder

Explore our comprehensive range of high quality products.

Promotions

See all of our current offers and say hello to our happy cells.

Productfinder

Explore our comprehensive range of high quality products.

Promotions

See all of our current offers and say hello to our happy cells.

Cookie Settings

IL-17 Secretion Assay – Cell Enrichment and Detection Kit (PE), human

IL-17 Secretion Assay – Cell Enrichment and Detection Kit (PE), human

Product overview

Product data and images for IL-17 Secretion Assay – Cell Enrichment and Detection Kit (PE), human

Figures

Figure 1

Figure 1

Figure 1

Figure 1

Figure 1

Specifications for IL-17 Secretion Assay – Cell Enrichment and Detection Kit (PE), human

Overview

Detailed product information

Background information

Columns

References for IL-17 Secretion Assay – Cell Enrichment and Detection Kit (PE), human

Publications

Data and images for IL-17 Secretion Assay – Cell Enrichment and Detection Kit (PE), human

Figures

Figure 1

Figure 1

Figure 1

Figure 1

Figure 1

Specifications for IL-17 Secretion Assay – Cell Enrichment and Detection Kit (PE), human

Overview

Detailed product information

Background information

Columns

References for IL-17 Secretion Assay – Cell Enrichment and Detection Kit (PE), human

Publications

Related products for IL-17 Secretion Assay – Cell Enrichment and Detection Kit (PE), human

7 products available | view all view less

CD154 Antibody, anti-human

CD161 Antibody, anti-human

CytoStim, human

130-097-196

TexMACS Medium

130-094-131

Naive CD4+ T Cell Isolation Kit II, human

130-094-125

CD4+ Effector Memory T Cell Isolation Kit, human

130-090-753

MACSmix Tube Rotator

Related products for
IL-17 Secretion Assay – Cell Enrichment and Detection Kit (PE), human

Naive CD4
⁺
T Cell Isolation Kit II
, human

CD4
⁺
Effector Memory T Cell Isolation Kit
, human