Clone:
REA829
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
ICFC, MICS, IF, IHC, MC
Alternative names:
IKZF2, ANF1A2, ZNF1A2, ZNFN1A2

Extended validation for Helios Antibody, anti-human/mouse, REAfinity™

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REA829
22F6++
736440-
Cells were incubated with an excess of purified unconjugated Helios (REA829) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Specifications for Helios Antibody, anti-human/mouse, REAfinity™

Overview

Clone REA829 recognizes mouse and human Helios, which is a zinc-finger transcription factor of the Ikaros family. As well as Ikaros and Aiolos, Helios functions as an important regulator of lymphoid development and differentiation and is involved in the control of gene expression. Helios is found on the earliest haematopoietic sites of the embryo, in adult haemopoietic stem cells, and on regulatory T cells.
Additional information: Clone REA829 displays negligible binding to Fc receptors.

Alternative names

IKZF2, ANF1A2, ZNF1A2, ZNFN1A2

Detailed product information

Technical specifications

CloneREA829
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (I), human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman, mouse, rat
Cross-reactivityrat
AntigenHelios
Alternative names of antigenIKZF2, ANF1A2, ZNF1A2, ZNFN1A2
Distribution of antigenT cells, hematopoietic stem and progenitor cells
Entrez Gene ID22807
RRIDAB_2651978, AB_2651979, AB_2651980, AB_2651981, AB_2651982, AB_2651983, AB_2651984, AB_2819659, AB_2651977

Resources for Helios Antibody, anti-human/mouse, REAfinity™

References for Helios Antibody, anti-human/mouse, REAfinity™

Publications

  1. Kelley, C. M. et al. (1998) Helios, a novel dimerization partner of Ikaros expressed in the earliest hematopoietic progenitors. Curr. Biol. 8(9): 508-515
  2. Rebollo, A. et al. (2003) Ikaros, Aiolos and Helios: Transcription regulators and lymphoid malignancies. Immunol. Cell Biol. 81: 171-175
  3. Thornton, A. M. et al. (2010)
    Expression of Helios, an Ikaros transcription factor family member, differentiates thymic-derived from peripherally induced Foxp3
    +
    T regulatory cells.
    Curr. Biol. 184: 3433-3441
  4. Gottschalk, R. A. et al. (2012)
    Expression of Helios in peripherally induced Foxp3
    +
    regulatory T cells.
    J. Immunol. 188: 976-980

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