Clone:
RB6-8C5
Type of antibody:
Primary antibodies
Isotype:
rat IgG2bκ
Applications:
FC, MICS, IF, IHC
Alternative names:
Ly-6G

Extended validation for Gr-1 Antibody, anti-mouse

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with RB6-8C5
REA526+
1A8+
REA810++
Cells were incubated with an excess of purified unconjugated Gr-1 (RB6-8C5) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Specifications for Gr-1 Antibody, anti-mouse

Overview

The mouse Anti-Gr-1 antibody reacts with Ly-6G, a 21–25 kDa, GPI-anchored cell surface protein, previously defined as the granulocyte-differentiation antigen-1 (Gr-1). The antibody has been shown to cross-react weakly with Ly-6C-transfected EL-4J cells. Cross-reactivity of Gr-1 with Ly-6C was not detected on hematopoietic cells that express Ly-6C and are negative for Ly-6G.
Gr-1 is expressed on mature granulocytes in bone marrow and peripheral tissues. The Anti-Gr-1 antibody also stains monocytes transiently during their differentiation in bone marrow and at low levels plasmacytoid dendritic cells in lymphoid tissues.

Alternative names

Ly-6G

Detailed product information

Technical specifications

CloneRB6-8C5
Clonalitymonoclonal
Isotyperat IgG2bκ
Isotype controlIsotype Control Antibody, rat IgG2b
Hostrat
Type of antibodyPrimary antibodies
Speciesmouse
AntigenGr-1
Alternative names of antigenLy-6G
Molecular mass of antigen [kDa]10
Entrez Gene ID546644
RRIDAB_2752206, AB_2751848, AB_2801792, AB_2659858, AB_2659859, AB_2659864, AB_2659865, AB_2659870

References for Gr-1 Antibody, anti-mouse

Publications

  1. Hestdal, K. et al. (1991) Characterization and regulation of RB6-8C5 antigen expression on murine bone marrow cells. J. Immunol. 147: 22-28
  2. Fleming, T. J. et al. (1993) Selective expression of Ly-6G on myeloid lineage cells in mouse bone marrow. RB6-8C5 mAb to granulocyte-differentiation antigen (Gr-1) detects members of the Ly-6 family. J. Immunol. 151: 2399-2408
  3. Nagendra, S. and Schlueter, A. J. (2004) Absence of cross-reactivity between murine Ly-6C and Ly-6G. Cytometry A 58: 195-200

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