CyTRAK Orange™ Staining Solution is an orange DNA stain which is cell permeable and intercalates double-stranded DNA (dsDNA) of living or fixed cells stoichiometrically.

Data and images for CyTRAK Orange™ Staining Solution

Figures

Figure 1

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Absorbance profile of CyTRAK Orange compared to eGFP and APC profile of CyTRAK Orange compared to eGFP and APC.

Figure 1

Absorbance profile of CyTRAK Orange compared to eGFP and APC profile of CyTRAK Orange compared to eGFP and APC.

Figure 2

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Emission profile of CyTRAK Orange compared to eGFP and Cy7.

Figure 2

Emission profile of CyTRAK Orange compared to eGFP and Cy7.

Figure 3

Nucleated cell gating by CyTRAK Orange intensity of intact, unlysed bone marrow with retained forward and side scatter characteristics.
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Figure 3

Nucleated cell gating by CyTRAK Orange intensity of intact, unlysed bone marrow with retained forward and side scatter characteristics.

Specifications for CyTRAK Orange™ Staining Solution

Overview

CyTRAK Orange™ Staining Solution is an orange DNA stain which is cell permeable and intercalates double-stranded DNA (dsDNA) of living or fixed cells stoichiometrically.

Detailed product information

CyTRAK Orange™ Staining Solution is an orange DNA stain which is cell permeable and intercalates double-stranded DNA (dsDNA) of living or fixed cells stoichiometrically. In addition to the preferentially stained nucleus the cytoplasma is also stained weakly. The fluorescence emission maximum for dsDNA‑bound CyTRAK Orange is 610 nm. With a broad excitation band with a maximum at 510 nm it can be excited efficiently by blue (488 nm) lasers.
CyTRAK Orange is very stable at room temperature and in ambient light with low photobleaching characteristics. The dye can be used in several flow cytometry applications:
  • Enumeration of nucleated cells
  • Nuclear counterstain in imaging and flow cytometry
  • Arrested/senescent cell identification
  • Nucl:cyto segmentation

Resources for CyTRAK Orange™ Staining Solution

Documents and Protocols

Brochures/posters

Viability dye guide

References for CyTRAK Orange™ Staining Solution

Publications

  1. Edward, R. (2009) Use of DNA-specific anthraquinone dyes to directly reveal cytoplasmic and nuclear boundaries in live and fixed cells. Mol. Cells 27(4): 391-396
  2. Edward, R. (2012) Red/far-red fluorescing DNA-specific anthraquinones for nucl:cyto segmentation and viability reporting in cell-based assays. Meth. Enzymol. 505: 23-45

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CyTRAK Orange™ Staining Solution

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