The Classical Monocyte Isolation Kit provides fast and consistent isolation of untouched classical ( CD14
++
CD16
) monocytes from peripheral blood mononuclear cells (PBMCs) for further functional studies. Depletion of non-monocytes is optimal and provides a highly pure population of label-free classical monocytes. This product includes an optional thrombocytes removal reagent to further reduce platelet contamination.

Data and images for Classical Monocyte Isolation Kit, human

Figures

Figure 1

CD14
+
CD16
cells were isolated from human peripheral blood mononuclear cells (PBMCs) by using the Classical Monocyte Isolation Kit, an LS Column, and a MidiMACS™ Separator. The cells were fluorescently stained with CD14-FITC and CD16-APC and analyzed by flow cytometry using the MACSQuant
®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
Before separation
CD14
+
CD16
cells
View details

Figure 1

CD14
+
CD16
cells were isolated from human peripheral blood mononuclear cells (PBMCs) by using the Classical Monocyte Isolation Kit, an LS Column, and a MidiMACS™ Separator. The cells were fluorescently stained with CD14-FITC and CD16-APC and analyzed by flow cytometry using the MACSQuant
®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
View details

Figure 1

CD14
+
CD16
cells were isolated from human peripheral blood mononuclear cells (PBMCs) by using the Classical Monocyte Isolation Kit, an LS Column, and a MidiMACS™ Separator. The cells were fluorescently stained with CD14-FITC and CD16-APC and analyzed by flow cytometry using the MACSQuant
®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Specifications for Classical Monocyte Isolation Kit, human

Overview

The Classical Monocyte Isolation Kit provides fast and consistent isolation of untouched classical ( CD14
++
CD16
) monocytes from peripheral blood mononuclear cells (PBMCs) for further functional studies. Depletion of non-monocytes is optimal and provides a highly pure population of label-free classical monocytes. This product includes an optional thrombocytes removal reagent to further reduce platelet contamination.

Detailed product information

Background information

Non-monocytes, such as T cells, NK cells, B cells, dendritic cells, and basophils are indirectly magnetically labeled using a cocktail of biotin-conjugated antibodies and Anti-Biotin MicroBeads. Isolation of highly pure unlabeled monocytes is achieved by depletion of the magnetically labeled cells.

Downstream applications

  • Functional studies on monocytes, in which any effect due to antibody binding to target cells should be avoided.
  • Studies on monocyte activation, differentiation, cytokine secretion etc.
  • Studies on compound or antigen uptake (e.g. phagocytosis) and antigen presentation by monocytes.
  • In vitro
    • differentiation of blood monocytes into dendritic cells or macrophages.

Columns

MS, LS, autoMACS
®
Columns, or Multi-24 Column Blocks

Related products for
Classical Monocyte Isolation Kit, human

10 products available | view all

Seems like you are coming from USA!
Do you want to visit our website in your country?