Name: CD90.2 MicroBeads, mouse
Availability: InStock

Product data and images for CD90.2 MicroBeads, mouse

Figures

Figure 1

A single-cell suspension from mouse spleen was prepared using the gentleMACS™ Dissociator. CD90.2

⁺

T cells were isolated from this single-cell suspension using the CD90.2 MicroBeads, an autoMACS

^®

Column, and autoMACS Pro Separator. Cells were fluorescently stained with REAfinity™ Antibodies CD45-FITC, CD45R(220)-PE-Vio

^®

770 and CD90.2-VioBlue

^®

and analyzed by flow cytometry using the MACSQuant

^®

Analyzer. Viable leukocytes were gated for analysis based on scatter signals, 7-AAD Staining Solution fluorescence, and CD45 expression.

Before separation

After separation

Figure 1

A single-cell suspension from mouse spleen was prepared using the gentleMACS™ Dissociator. CD90.2

⁺

T cells were isolated from this single-cell suspension using the CD90.2 MicroBeads, an autoMACS

^®

Column, and autoMACS Pro Separator. Cells were fluorescently stained with REAfinity™ Antibodies CD45-FITC, CD45R(220)-PE-Vio

^®

770 and CD90.2-VioBlue

^®

and analyzed by flow cytometry using the MACSQuant

^®

Analyzer. Viable leukocytes were gated for analysis based on scatter signals, 7-AAD Staining Solution fluorescence, and CD45 expression.

Figure 1

A single-cell suspension from mouse spleen was prepared using the gentleMACS™ Dissociator. CD90.2

⁺

T cells were isolated from this single-cell suspension using the CD90.2 MicroBeads, an autoMACS

^®

Column, and autoMACS Pro Separator. Cells were fluorescently stained with REAfinity™ Antibodies CD45-FITC, CD45R(220)-PE-Vio

^®

770 and CD90.2-VioBlue

^®

and analyzed by flow cytometry using the MACSQuant

^®

Analyzer. Viable leukocytes were gated for analysis based on scatter signals, 7-AAD Staining Solution fluorescence, and CD45 expression.

Specifications for CD90.2 MicroBeads, mouse

Overview

CD90.2 MicroBeads were developed for positive selection or depletion of mouse T cells from single-cell suspensions of lymphoid and non-lymphoid tissues or from peripheral blood.

Detailed product information

Background information

The mouse CD90.2 alloantigen, also known as Thy1.2, is a pan T cell marker for the most common inbred mouse strains. These strains include BALB/c, DBA, CBA/J, C3H, C57BL/6, NZB/-, S3L and others. CD90.2 is expressed on thymocytes, peripheral T cells, on some intraepithelial T cells, and at lower levels on early hematopoietic stem cells in bone marrow.

The mouse CD90.2 antibody does not cross-react with CD90.1 (Thy1.1).

Downstream applications

T cells isolated with CD90.2 MicroBeads were used to analyze cytokine expression in a mouse model of lung fibrosis or after adoptive transfer of T cells from wild type and knock-out mice into immunodeficient mice.
Furthermore, CD90.2 MicroBeads were used for the isolation of tumor-infiltrating T cells to evaluate their immunotherapeutic potential or functional properties.

Columns

For positive selection: MS, LS, or autoMACS

^®

Columns. For depletion: LD or autoMACS Columns.

Resources for CD90.2 MicroBeads, mouse

Documents and Protocols

Scientific posters

New generation CD90.1 and CD90.2 MicroBeads - fast isolation of adoptively transferred T cells from mouse

Data and images

Data and images for CD90.2 MicroBeads, mouse

Figures

Figure 1

A single-cell suspension from mouse spleen was prepared using the gentleMACS™ Dissociator. CD90.2

⁺

T cells were isolated from this single-cell suspension using the CD90.2 MicroBeads, an autoMACS

^®

Column, and autoMACS Pro Separator. Cells were fluorescently stained with REAfinity™ Antibodies CD45-FITC, CD45R(220)-PE-Vio

^®

770 and CD90.2-VioBlue

^®

and analyzed by flow cytometry using the MACSQuant

^®

Analyzer. Viable leukocytes were gated for analysis based on scatter signals, 7-AAD Staining Solution fluorescence, and CD45 expression.

Before separation

After separation

Figure 1

A single-cell suspension from mouse spleen was prepared using the gentleMACS™ Dissociator. CD90.2

⁺

T cells were isolated from this single-cell suspension using the CD90.2 MicroBeads, an autoMACS

^®

Column, and autoMACS Pro Separator. Cells were fluorescently stained with REAfinity™ Antibodies CD45-FITC, CD45R(220)-PE-Vio

^®

770 and CD90.2-VioBlue

^®

and analyzed by flow cytometry using the MACSQuant

^®

Analyzer. Viable leukocytes were gated for analysis based on scatter signals, 7-AAD Staining Solution fluorescence, and CD45 expression.

Figure 1

A single-cell suspension from mouse spleen was prepared using the gentleMACS™ Dissociator. CD90.2

⁺

T cells were isolated from this single-cell suspension using the CD90.2 MicroBeads, an autoMACS

^®

Column, and autoMACS Pro Separator. Cells were fluorescently stained with REAfinity™ Antibodies CD45-FITC, CD45R(220)-PE-Vio

^®

770 and CD90.2-VioBlue

^®

and analyzed by flow cytometry using the MACSQuant

^®

Analyzer. Viable leukocytes were gated for analysis based on scatter signals, 7-AAD Staining Solution fluorescence, and CD45 expression.

Specifications

Specifications for CD90.2 MicroBeads, mouse

Overview

CD90.2 MicroBeads were developed for positive selection or depletion of mouse T cells from single-cell suspensions of lymphoid and non-lymphoid tissues or from peripheral blood.

Detailed product information

Background information

The mouse CD90.2 antibody does not cross-react with CD90.1 (Thy1.1).

Downstream applications

T cells isolated with CD90.2 MicroBeads were used to analyze cytokine expression in a mouse model of lung fibrosis or after adoptive transfer of T cells from wild type and knock-out mice into immunodeficient mice.
Furthermore, CD90.2 MicroBeads were used for the isolation of tumor-infiltrating T cells to evaluate their immunotherapeutic potential or functional properties.

Columns

For positive selection: MS, LS, or autoMACS

^®

Columns. For depletion: LD or autoMACS Columns.

Resources

Resources for CD90.2 MicroBeads, mouse

Documents and Protocols

Scientific posters

Contact

Contact

CD90.2 MicroBeads, mouse

CD90.2 MicroBeads, mouse

Product data and images for CD90.2 MicroBeads, mouse

Figures

Figure 1

Figure 1

Figure 1

Specifications for CD90.2 MicroBeads, mouse

Overview

Detailed product information

Background information

Downstream applications

Columns

Resources for CD90.2 MicroBeads, mouse

Documents and Protocols

New generation CD90.1 and CD90.2 MicroBeads - fast isolation of adoptively transferred T cells from mouse

Data and images

Data and images for CD90.2 MicroBeads, mouse

Figures

Figure 1

Figure 1

Figure 1

Specifications

Specifications for CD90.2 MicroBeads, mouse

Overview

Detailed product information

Background information

Downstream applications

Columns

Resources

Resources for CD90.2 MicroBeads, mouse

Documents and Protocols

New generation CD90.1 and CD90.2 MicroBeads - fast isolation of adoptively transferred T cells from mouse

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CD90.2 MicroBeads, mouse

CD90.2 MicroBeads, mouse

Product data and images for CD90.2 MicroBeads, mouse

Figures

Figure 1