Clone:
30-H12
Type of antibody:
Primary antibodies
Isotype:
rat IgG2bκ
Applications:
FC, MICS, IF, IHC
Alternative names:
THY1, T25, Thy-1, Thy-1.2

Extended validation for CD90.2 Antibody, anti-mouse

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with 30-H12
REA1167++
53-2.1++
Cells were incubated with an excess of purified unconjugated CD90.2 (30-H12) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using CD90.2 (30-H12). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD90.2 (30-H12). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using CD90.2 (30-H12). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD90.2 Antibody, anti-mouse

Overview

Clone 30-H12 recognizes the mouse CD90.2 (Thy1.2) antigen which is expressed at high levels on thymocytes and peripheral T cells in lymphoid organs and blood as well as on retinal ganglion cells. The CD90.2 antibody clone 30-H12 reacts with the Thy1.2 alloantigen, which is a pan–T cell marker for the most common inbred mouse strain. These strains include BALB/c, DBA, CBA/J, C3H, C57BL/6, NZB/-, S3L, and others. It does not cross-react with Thy1.1. CD90.2 is also expressed at lower levels on early hematopoietic stem cells in the bone marrow, on interepithelial cells (dendritic epidermal T cells) in skin, and on neurons.

Alternative names

THY1, T25, Thy-1, Thy-1.2

Detailed product information

Technical specifications

Clone30-H12
Clonalitymonoclonal
Isotyperat IgG2bκ
Isotype controlIsotype Control Antibody, rat IgG2b
Hostrat
Type of antibodyPrimary antibodies
Speciesmouse
AntigenCD90.2
Alternative names of antigenTHY1, T25, Thy-1, Thy-1.2
Molecular mass of antigen [kDa]13
Distribution of antigenendothelial cells, fibroblasts, neurons, T cells, stem cells, leukocytes, lymphocytes, cancer stem cells, hematopoietic stem and progenitor cells, leukemia cells, mesenchymal stem cells, ES and iPS cells, thymocytes, brain
Entrez Gene ID21838
RRIDAB_2751997, AB_2876917, AB_2659874, AB_2659875, AB_2659877, AB_2659878, AB_2659879, AB_2650534, AB_2659880, AB_2659881, AB_2752033

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