CD8
⁺
Memory T Cell Isolation Kit
, human

Name: CD8+ Memory T Cell Isolation Kit, human
Availability: InStock

CD8
⁺
Memory T Cell Isolation Kit
, human

The CD8

⁺

Memory T Cell Isolation Kit was developed for the isolation of untouched CD8

⁺

CD45RO

⁺

CD45RA

^–

CD56

^–

CD57

^–

memory T cells from human PBMCs.

Data and images
Specifications

Product data and images for CD8
⁺
Memory T Cell Isolation Kit
, human

Figures

Figure 1

Untouched CD8

⁺

memory T cells were isolated from human PBMCs using the CD8

⁺

Memory T Cell Isolation Kit, an LS Column, and a MidiMACS™ Separator. Cells were stained with CD45RA-FITC, CD57-PE, CD56-PE-Cy7, and CD8-APC.

Unseparated fraction

Gated on viable CD8 ⁺ CD56 ^– cells.
View details CD8 ⁺ Memory T Cell Isolation Kit , human Figure 1 Untouched CD8 ⁺ memory T cells were isolated from human PBMCs using the CD8 ⁺ Memory T Cell Isolation Kit, an LS Column, and a MidiMACS™ Separator. Cells were stained with CD45RA-FITC, CD57-PE, CD56-PE-Cy7, and CD8-APC.	View details CD8 ⁺ Memory T Cell Isolation Kit , human Figure 1 Untouched CD8 ⁺ memory T cells were isolated from human PBMCs using the CD8 ⁺ Memory T Cell Isolation Kit, an LS Column, and a MidiMACS™ Separator. Cells were stained with CD45RA-FITC, CD57-PE, CD56-PE-Cy7, and CD8-APC.
Isolated CD8⁺ memory T cells

Gated on viable CD8 ⁺ CD56 ^– cells.
View details CD8 ⁺ Memory T Cell Isolation Kit , human Figure 1 Untouched CD8 ⁺ memory T cells were isolated from human PBMCs using the CD8 ⁺ Memory T Cell Isolation Kit, an LS Column, and a MidiMACS™ Separator. Cells were stained with CD45RA-FITC, CD57-PE, CD56-PE-Cy7, and CD8-APC.	View details CD8 ⁺ Memory T Cell Isolation Kit , human Figure 1 Untouched CD8 ⁺ memory T cells were isolated from human PBMCs using the CD8 ⁺ Memory T Cell Isolation Kit, an LS Column, and a MidiMACS™ Separator. Cells were stained with CD45RA-FITC, CD57-PE, CD56-PE-Cy7, and CD8-APC.

Specifications for CD8
⁺
Memory T Cell Isolation Kit
, human

Overview

The CD8

⁺

Memory T Cell Isolation Kit was developed for the isolation of untouched CD8

⁺

CD45RO

⁺

CD45RA

^–

CD56

^–

CD57

^–

memory T cells from human PBMCs.

Detailed product information

Detailed separation procedure

Non-target cells are indirectly magnetically labeled by using a cocktail of biotin-conjugated antibodies against CD4, CD11c, CD14, CD15, CD16, CD19, CD34, CD36, CD45RA, CD56, CD57, CD61, CD123, CD141, TCR γ/δ, and CD235a (glycophorin A), and Anti-Biotin MicroBeads. Isolation of highly pure CD8

⁺

memory T cells is achieved by depletion of the magnetically labeled cells.

Downstream applications

CD8

⁺

memory T cells isolated with the CD8

⁺

Memory T Cell Isolation Kit are used when direct labeling of cell surface molecules could interfere with downstream applications, e.g., in studies on memory T cell activation. Untouched CD8

⁺

memory T cells can also be used for phenotypical and functional studies.

Columns

MS, LS, XS, or autoMACS

^®

Columns.

Data and images