The CD8
+
Memory T Cell Isolation Kit was developed for the isolation of untouched CD8
+
CD45RO
+
CD45RA
CD56
CD57
memory T cells from human PBMCs.

Data and images for
CD8
+
Memory T Cell Isolation Kit
, human

Figures

Figure 1

Untouched CD8
+
memory T cells were isolated from human PBMCs using the CD8
+
Memory T Cell Isolation Kit, an LS Column, and a MidiMACS™ Separator. Cells were stained with CD45RA-FITC, CD57-PE, CD56-PE-Cy7, and CD8-APC.
Unseparated fraction
Gated on viable CD8
+
CD56
cells.
View details

Figure 1

Untouched CD8
+
memory T cells were isolated from human PBMCs using the CD8
+
Memory T Cell Isolation Kit, an LS Column, and a MidiMACS™ Separator. Cells were stained with CD45RA-FITC, CD57-PE, CD56-PE-Cy7, and CD8-APC.
View details

Figure 1

Untouched CD8
+
memory T cells were isolated from human PBMCs using the CD8
+
Memory T Cell Isolation Kit, an LS Column, and a MidiMACS™ Separator. Cells were stained with CD45RA-FITC, CD57-PE, CD56-PE-Cy7, and CD8-APC.
Isolated CD8+ memory T cells
Gated on viable CD8
+
CD56
cells.
View details

Figure 1

Untouched CD8
+
memory T cells were isolated from human PBMCs using the CD8
+
Memory T Cell Isolation Kit, an LS Column, and a MidiMACS™ Separator. Cells were stained with CD45RA-FITC, CD57-PE, CD56-PE-Cy7, and CD8-APC.
View details

Figure 1

Untouched CD8
+
memory T cells were isolated from human PBMCs using the CD8
+
Memory T Cell Isolation Kit, an LS Column, and a MidiMACS™ Separator. Cells were stained with CD45RA-FITC, CD57-PE, CD56-PE-Cy7, and CD8-APC.

Specifications for
CD8
+
Memory T Cell Isolation Kit
, human

Overview

The CD8
+
Memory T Cell Isolation Kit was developed for the isolation of untouched CD8
+
CD45RO
+
CD45RA
CD56
CD57
memory T cells from human PBMCs.

Detailed product information

Detailed separation procedure

Non-target cells are indirectly magnetically labeled by using a cocktail of biotin-conjugated antibodies against CD4, CD11c, CD14, CD15, CD16, CD19, CD34, CD36, CD45RA, CD56, CD57, CD61, CD123, CD141, TCR γ/δ, and CD235a (glycophorin A), and Anti-Biotin MicroBeads. Isolation of highly pure CD8
+
memory T cells is achieved by depletion of the magnetically labeled cells.

Downstream applications

CD8
+
memory T cells isolated with the CD8
+
Memory T Cell Isolation Kit are used when direct labeling of cell surface molecules could interfere with downstream applications, e.g., in studies on memory T cell activation. Untouched CD8
+
memory T cells can also be used for phenotypical and functional studies.

Columns

MS, LS, XS, or autoMACS
®
Columns.

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CD8
+
Memory T Cell Isolation Kit
, human

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