The CD28 MicroBead Kit was developed for the positive selection or depletion of CD28
+
cells from human PBMCs.

Data and images for CD28 MicroBead Kit, human

Figures

Figure 1

CD28
+
cells were fluorescently and magnetically labeled using the CD28 MicroBead Kit. Labeled cells were separated from human PBMCs using an MS Column and a MiniMACS™ Separator. Cells were additionally stained with CD8-FITC.
Unseparated fraction
CD28
+
cells
View details

Figure 1

CD28
+
cells were fluorescently and magnetically labeled using the CD28 MicroBead Kit. Labeled cells were separated from human PBMCs using an MS Column and a MiniMACS™ Separator. Cells were additionally stained with CD8-FITC.
View details

Figure 1

CD28
+
cells were fluorescently and magnetically labeled using the CD28 MicroBead Kit. Labeled cells were separated from human PBMCs using an MS Column and a MiniMACS™ Separator. Cells were additionally stained with CD8-FITC.

Specifications for CD28 MicroBead Kit, human

Overview

The CD28 MicroBead Kit was developed for the positive selection or depletion of CD28
+
cells from human PBMCs.

Detailed product information

Background information

The CD28
+
cells are indirectly magnetically labeled with CD28-PE and Anti-PE MicroBeads.
The CD28 antigen is a type I trans‑membrane protein, highly expressed on most CD4
+
T cells, many CD8
+
T cells, and on malignant plasma cells (myeloma cells). Ligation of CD28 with CD80 (B7-1) or CD86 (B7-2) on target cells provides a critical costimulatory signal for T cell activation. Blockade of CD28-B7 interaction leads to an inhibition of immune responses both
in vivo
and
in vitro
.

Downstream applications

The CD28 MicroBead Kit was developed for the isolation of CD28
+
cells from PBMCs, e.g., for phenotypical or functional characterization.
CD28
+
T cell subsets can be isolated in combination with kits for untouched T cell isolation, e.g., the CD4
+
T Cell Isolation Kit or the CD8
+
T Cell Isolation Kit.

Columns

For positive selection: MS, LS, XS, or autoMACS
®
Columns. For depletion: LD, D, or autoMACS Columns.

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