Name: CD271 MicroBead Kits, human
Availability: InStock

Product data and images for CD271 MicroBead Kits, human

Figures

Figure 1

Enrichment of CD271

⁺

mesenchymal stromal cells (MSCs) from bone marrow mononuclear cells (BM-MNCs) using the CD271 MicroBead Kit, an MS Column, and a MiniMACS™ Separator. Cells were stained with CD271 antibodies conjugated to PE as well as CD45-FITC and analyzed by flow cytometry using the MACSQuant

^®

Analyzer.

BM-MNCs before separation	CD271 ⁺ cells

Specifications for CD271 MicroBead Kits, human

Overview

CD271 MicroBead Kits have been developed for the positive selection or depletion of cells expressing CD271 (LNGFR). They are ideally suited for the isolation of CD271

⁺

mesenchymal stromal cells (MSCs) from human tissues, including bone marrow and lipoaspirate. In addition, CD271 MicroBead Kits have been used successfully for the isolation of MSCs from bone marrow of monkey, goat, dog, pig, and sheep.

Detailed product information

Background information

CD271, also known as LNGFR (low-affinity nerve growth factor receptor) or p75NTR, belongs to the low-affinity neurotrophin receptor and the tumor necrosis factor receptor superfamily.

CD271 is a well-known marker on mesenchymal stem cells, also known as mesenchymal stromal cells (MSCs), from bone marrow aspirate

^1–4

or lipoaspirate

⁵

. After separation colony-forming unit fibroblast (CFU-F) activity was found only in the CD271

⁺

cell fraction, and not in the CD271

^–

population

^6–8

. Isolated CD271

⁺

cells have a higher proliferative capacity in comparison to MSCs isolated by plastic adherence

^6,8,9

. Secretion of growth factors was significantly higher in the separated CD271

⁺

MSCs

⁷

CD271 (LNGFR) was initially described to be expressed on cells of the central and peripheral nervous system nervous system and was suggested to be involved in the development, survival, and differentiation of neural cells

¹⁰

. CD271

⁺

neural crest progenitor cells can be efficiently isolated with Neural Crest Stem Cell MicroBeads.

Cross-reactivity

The CD271 antibody shows cross-reactivity with monkey, goat, dog, pig, and sheep

¹¹

Columns

MS, LS, XS, or autoMACS

^®

Columns.

Resources for CD271 MicroBead Kits, human

Documents and Protocols

App notes/customer reports

Clinical-scale isolation of mesenchymal stromal cells from bone marrow according to their CD271 expression. Godthardt and Schmitz (2012) MACS&more 14(2).

Brochures/posters

Workflow for human mesenchymal stem or stromal cells

Protocols

Isolation of CD271+ MSCs/ADSCs from human lipoaspirate

Scientific posters

Clinical-scale isolation of mesenchymal stromal cells from bone marrow with the CliniMACS® System and CD271 antibody–conjugated MicroBeads.

Scientific posters

Adipose-derived marrow stromal cells from lipoaspirate are CD271-positive

References for CD271 MicroBead Kits, human

Publications

Quirici, N. et al. (2002) Isolation of bone marrow mesenchymal stem cells by anti-nerve growth factor receptor antibodies. Exp. Hematol. 30: 783-791
Jones et al. (2006) Optimization of a flow cytometry-based protocol for detection and phenotypic characterization of multipotent mesenchymal stromal cells from human bone marrow. Cytometry B Clin. Cytom. 70: 391-399
Poloni et al. (2009)
Selection of CD271
⁺
cells and human AB serum allows a large expansion of mesenchymal stromal cells from human bone marrow.
Cytotherapy 11: 153-162
Thomson, T. M. et al. (1988) Expression of human nerve growth factor receptor on cells derived from all three germ layers. Exp. Cell Res. 174: 533-539
Jarocha, D. et al. (2008)
Adventage of mesenchymal stem cells (MSC) expansion directly from purified bone marrow CD105
⁺
and CD271
⁺
cells.
Folia Histochem. Cytobiol. 46: 307-314
Kuci et al. (2010) CD271 antigen defines a subset of multipotent stromal cells with immunosuppressive and lymphohematopoietic engraftment-promoting properties. Haematologica 95: 651-659
Meyerrose, T. E. et al. (2007)
In vivo
distribution of human adipose-derived mesenchymal stem cells in novel xenotransplantation models.
Stem Cells 25: 220-227
Rozemuller, H. et al. (2010) Prospective isolation of mesenchymal stem cells from multiple mammalian species using cross-reacting anti-human monoclonal antibodies. Stem Cells Dev. 19: 1911-1921
Jones, E.A. et al. (2007) Purification of proliferative and multipotential marrow stromal cells (MSCs) from bone marrow aspirate by selection for CD271 (LNGFR) expression. MACS&more 11(1): 22-25
Jones, E.A. et al. (2004) 4th Annual Conference on Mesenchymal and Nonhematopoietic stem cells : abstract
Jones, E.A. et al. (2004) ASH meeting 29(abstract): 2337

Data and images

Data and images for CD271 MicroBead Kits, human

Figures

Figure 1

Enrichment of CD271

⁺

^®

Analyzer.

BM-MNCs before separation	CD271 ⁺ cells

Specifications

Specifications for CD271 MicroBead Kits, human

Overview

CD271 MicroBead Kits have been developed for the positive selection or depletion of cells expressing CD271 (LNGFR). They are ideally suited for the isolation of CD271

⁺

Detailed product information

Background information

CD271, also known as LNGFR (low-affinity nerve growth factor receptor) or p75NTR, belongs to the low-affinity neurotrophin receptor and the tumor necrosis factor receptor superfamily.

CD271 is a well-known marker on mesenchymal stem cells, also known as mesenchymal stromal cells (MSCs), from bone marrow aspirate

^1–4

or lipoaspirate

⁵

. After separation colony-forming unit fibroblast (CFU-F) activity was found only in the CD271

⁺

cell fraction, and not in the CD271

^–

population

^6–8

. Isolated CD271

⁺

cells have a higher proliferative capacity in comparison to MSCs isolated by plastic adherence

^6,8,9

. Secretion of growth factors was significantly higher in the separated CD271

⁺

MSCs

⁷

¹⁰

. CD271

⁺

neural crest progenitor cells can be efficiently isolated with Neural Crest Stem Cell MicroBeads.

Cross-reactivity

The CD271 antibody shows cross-reactivity with monkey, goat, dog, pig, and sheep

¹¹

Columns

MS, LS, XS, or autoMACS

^®

Columns.

Resources

Resources for CD271 MicroBead Kits, human

Documents and Protocols

App notes/customer reports

Clinical-scale isolation of mesenchymal stromal cells from bone marrow according to their CD271 expression. Godthardt and Schmitz (2012) MACS&more 14(2).

Brochures/posters

Workflow for human mesenchymal stem or stromal cells

Protocols

Isolation of CD271+ MSCs/ADSCs from human lipoaspirate

Scientific posters

Clinical-scale isolation of mesenchymal stromal cells from bone marrow with the CliniMACS® System and CD271 antibody–conjugated MicroBeads.

Scientific posters

Adipose-derived marrow stromal cells from lipoaspirate are CD271-positive

References

References for CD271 MicroBead Kits, human

Publications

Quirici, N. et al. (2002) Isolation of bone marrow mesenchymal stem cells by anti-nerve growth factor receptor antibodies. Exp. Hematol. 30: 783-791
Jones et al. (2006) Optimization of a flow cytometry-based protocol for detection and phenotypic characterization of multipotent mesenchymal stromal cells from human bone marrow. Cytometry B Clin. Cytom. 70: 391-399
Poloni et al. (2009)
Selection of CD271
⁺
cells and human AB serum allows a large expansion of mesenchymal stromal cells from human bone marrow.
Cytotherapy 11: 153-162
Thomson, T. M. et al. (1988) Expression of human nerve growth factor receptor on cells derived from all three germ layers. Exp. Cell Res. 174: 533-539
Jarocha, D. et al. (2008)
Adventage of mesenchymal stem cells (MSC) expansion directly from purified bone marrow CD105
⁺
and CD271
⁺
cells.
Folia Histochem. Cytobiol. 46: 307-314
Kuci et al. (2010) CD271 antigen defines a subset of multipotent stromal cells with immunosuppressive and lymphohematopoietic engraftment-promoting properties. Haematologica 95: 651-659
Meyerrose, T. E. et al. (2007)
In vivo
distribution of human adipose-derived mesenchymal stem cells in novel xenotransplantation models.
Stem Cells 25: 220-227
Rozemuller, H. et al. (2010) Prospective isolation of mesenchymal stem cells from multiple mammalian species using cross-reacting anti-human monoclonal antibodies. Stem Cells Dev. 19: 1911-1921
Jones, E.A. et al. (2007) Purification of proliferative and multipotential marrow stromal cells (MSCs) from bone marrow aspirate by selection for CD271 (LNGFR) expression. MACS&more 11(1): 22-25
Jones, E.A. et al. (2004) 4th Annual Conference on Mesenchymal and Nonhematopoietic stem cells : abstract
Jones, E.A. et al. (2004) ASH meeting 29(abstract): 2337

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CD271 MicroBead Kits, human

CD271 MicroBead Kits, human

Product overview

Product data and images for CD271 MicroBead Kits, human

Figures

Figure 1

Specifications for CD271 MicroBead Kits, human

Overview

Detailed product information

Background information

Cross-reactivity

Columns

Resources for CD271 MicroBead Kits, human

Documents and Protocols

References for CD271 MicroBead Kits, human

Publications

Data and images for CD271 MicroBead Kits, human

Figures

Figure 1

Specifications for CD271 MicroBead Kits, human

Overview

Detailed product information

Background information

Cross-reactivity

Columns

Resources for CD271 MicroBead Kits, human

Documents and Protocols

References for CD271 MicroBead Kits, human

Publications

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