Clone:
ME20.4-1.H4
Type of antibody:
Primary antibodies
Isotype:
mouse IgG1κ
Applications:
FC, MICS, IF, IHC
Alternative names:
NGFR, Gp80-LNGFR, TNFRSF16, p75(NTR), p75NTR, p75NGFR, NGFR (p75)

Extended validation for CD271 (LNGFR) Antibody, anti-human

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with ME20.4-1H4
REA844++
ME20.4++
C40-1457+
REA648 (h/m)-
REAL709+
Cells were incubated with an excess of purified unconjugated CD271 (LNGFR) (ME20.4-1.H4) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for CD271 (LNGFR). CD271 (LNGFR) trasfected positive Jurkat cells and negative peripheral blood monoclear cells (PBMCs) were stained with CD271 (LNGFR) antibodies and with a suitable counterstaining. As a control, CD271 (LNGFR) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD271 (LNGFR). CD271 (LNGFR) trasfected positive Jurkat cells and negative peripheral blood monoclear cells (PBMCs) were stained with CD271 (LNGFR) antibodies and with a suitable counterstaining. As a control, CD271 (LNGFR) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD271 (LNGFR). CD271 (LNGFR) trasfected positive Jurkat cells and negative peripheral blood monoclear cells (PBMCs) were stained with CD271 (LNGFR) antibodies and with a suitable counterstaining. As a control, CD271 (LNGFR) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD271 (LNGFR). CD271 (LNGFR) trasfected positive Jurkat cells and negative peripheral blood monoclear cells (PBMCs) were stained with CD271 (LNGFR) antibodies and with a suitable counterstaining. As a control, CD271 (LNGFR) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD271 (LNGFR). CD271 (LNGFR) trasfected positive Jurkat cells and negative peripheral blood monoclear cells (PBMCs) were stained with CD271 (LNGFR) antibodies and with a suitable counterstaining. As a control, CD271 (LNGFR) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD271 (LNGFR). CD271 (LNGFR) trasfected positive Jurkat cells and negative peripheral blood monoclear cells (PBMCs) were stained with CD271 (LNGFR) antibodies and with a suitable counterstaining. As a control, CD271 (LNGFR) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD271 (LNGFR). CD271 (LNGFR) trasfected positive Jurkat cells and negative peripheral blood monoclear cells (PBMCs) were stained with CD271 (LNGFR) antibodies and with a suitable counterstaining. As a control, CD271 (LNGFR) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for CD271 (LNGFR). CD271 (LNGFR) trasfected positive Jurkat cells and negative peripheral blood monoclear cells (PBMCs) were stained with CD271 (LNGFR) antibodies and with a suitable counterstaining. As a control, CD271 (LNGFR) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Specifications for CD271 (LNGFR) Antibody, anti-human

Overview

Clone ME20.4-1.H4 recognizes the CD271 antigen, also known as low-affinity nerve growth factor receptor (LNGFR) or neurotrophin receptor (p75 NTR). CD271 belongs to the low-affinity neurotrophin receptors and the tumor necrosis factor receptor superfamily. Initially the human CD271 (LNGFR) was described to be expressed on cells of the central and peripheral nervous system, and was suggested to be involved in the development, survival, and differentiation of cells.
1
CD271 (LNGFR) is expressed on
  • Mesenchymal stem/stromal cells (MSCs)2,3,4
  • Follicular dendritic cells5
  • Mesenchymal cells involved in mesenchymal-epithelial interactions6
  • Autonomic and sensory Neurons7
  • Oligodendrocytes8
  • Astrocytes9
  • Schwann cells10,11
  • Neural crest stem cells12

Alternative names

NGFR, Gp80-LNGFR, TNFRSF16, p75(NTR), p75NTR, p75NGFR, NGFR (p75)

Detailed product information

Technical specifications

CloneME20.4-1.H4
Clonalitymonoclonal
Isotypemouse IgG1κ
Isotype controlIsotype Control Antibody, mouse IgG1
Hostmouse
Type of antibodyPrimary antibodies
Specieshuman, non-human primate, other
Cross-reactivitymonkey, goat, dog, pig, sheep
AntigenCD271 (LNGFR)
Alternative names of antigenNGFR, Gp80-LNGFR, TNFRSF16, p75(NTR), p75NTR, p75NGFR, NGFR (p75)
Molecular mass of antigen [kDa]43
Distribution of antigenB cells, stem cells, dendritic cells, monocytes, neurons, cancer stem cells, mesenchymal stem cells, bone marrow
Entrez Gene ID4804
RRIDAB_2733631, AB_2734063, AB_2734064, AB_2733794, AB_2733795, AB_2733362, AB_2733363, AB_2733936, AB_2733937, AB_2733219, AB_2733220, AB_2733512, AB_2733513, AB_2733630

References for CD271 (LNGFR) Antibody, anti-human

Publications

  1. Caneva, L. et al. (1995) Immuno-electron microscopy characterization of human bone marrow stromal cells with anti-NGFR antibodies. Blood Cells Mol. Dis. 21: 73-85
  2. Quirici, N. et al. (2002) Isolation of bone marrow mesenchymal stem cells by anti-nerve growth factor receptor antibodies. Exp. Hematol. 30: 783-791
  3. Casaccia-Bonnefil, P. et al. (1996) Death of oligodendrocytes mediated by the interaction of nerve growth factor with its receptor p75. Nature 383: 716-719
  4. DiStefano, P. S. and Johnson, E. M. Jr. (1988) Identification of a truncated form of the nerve growth factor receptor. Proc. Natl. Acad. Sci. U.S.A. 85: 270-274
  5. Civenni, G. et al. (2011) Human CD271-positive melanoma stem cells associated with metastasis establish tumor heterogeneity and long-term growth. Cancer Res. 71(8): 3098-57363109
  6. Huber, L. J. and Chao, M. V. (1995) Mesenchymal and neuronal cell expression of the p75 neurotrophin receptor gene occur by different mechanisms. Dev. Biol. 167: 227-238
  7. Chalazonitis, A. et al. (1998)
    Age-dependent differences in the effects of GDNF and NT-3 on the development of neurons and glia from neural crest-derived precursors immunoselected from the fetal rat gut: expression of GFRalpha-1
    in vitro
    and
    in vivo
    .
    Dev. Biol. 204(2): 385-406
  8. Jones, E. A. et al. (2002) Isolation and characterization of bone marrow multipotential mesenchymal progenitor cells. Arthritis Rheum 46: 3349-3360
  9. Kashiba, H. et al. (1995) Coexpression of trk family members and low-affinity neurotrophin receptors in rat dorsal root ganglion neurons. Brain Res. Mol. Brain Res. 30: 158-164
  10. Rudge, J. S. et al. (1994) Neurotrophic factor receptors and their signal transduction capabilities in rat astrocytes. Eur. J. Neurosci. 6: 693-705
  11. Thomson, T. M. et al. (1988) Expression of human nerve growth factor receptor on cells derived from all three germ layers. Exp. Cell Res. 174: 533-539
  12. Pezzati, P. et al. (1992) Expression of nerve growth factor receptor immunoreactivity on follicular dendritic cells from human mucosa associated lymphoid tissues. Immunology 76: 485-490
  13. Rozemuller, H. et al. (2010) Prospective isolation of mesenchymal stem cells from multiple mammalian species using cross-reacting anti-human monoclonal antibodies. Stem Cells Dev. 19: 1911-1921
  14. Vroemen, M. and Weidner, N. (2003) Purification of Schwann cells by selection of p75 low affinity nerve growth factor receptor expressing cells from adult peripheral nerve. J. Neurosci. Methods 124: 135-143

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