The CD25 MicroBead Kit has been developed for the positive selection or depletion of mouse CD25
+
cells from single-cell suspensions of spleen and lymph nodes.

Data and images for CD25 MicroBead Kit, mouse

Figures

Figure 1

Isolation of CD25
+
cells from a mouse spleen cell suspension using the CD25 MicroBead Kit, two MS Columns, and a MiniMACS™ Separator.
Spleen cells before separation
CD25
-
cells after separation
View details

Figure 1

Isolation of CD25
+
cells from a mouse spleen cell suspension using the CD25 MicroBead Kit, two MS Columns, and a MiniMACS™ Separator.
View details

Figure 1

Isolation of CD25
+
cells from a mouse spleen cell suspension using the CD25 MicroBead Kit, two MS Columns, and a MiniMACS™ Separator.
CD25
+
cells after separation
View details

Figure 1

Isolation of CD25
+
cells from a mouse spleen cell suspension using the CD25 MicroBead Kit, two MS Columns, and a MiniMACS™ Separator.

Specifications for CD25 MicroBead Kit, mouse

Overview

The CD25 MicroBead Kit has been developed for the positive selection or depletion of mouse CD25
+
cells from single-cell suspensions of spleen and lymph nodes.

Detailed product information

Background information

For separation, CD25
+
cells are indirectly magnetically labeled with CD25-PE and Anti-PE MicroBeads. CD25 is the IL-2R α-chain, which combined with the β-chain (CD122) and the γ-chain (CD132) forms the functional IL-2R complex. Binding of CD25-PE does not inhibit the adhesion of IL-2 to its receptor. IL-2Rα is expressed on activated T and B cells, to a lower extent on activated dendritic cells, on regulatory T cells, in the early stage of thymic T cell development (CD4
-
CD8
-
), and transiently during B cell ontogeny. NK cells and naive T and B cells do not express IL2-Rα.

Downstream applications

CD25
+
cells isolated by using the CD25 MicroBead Kit are suitable for
in vitro
analysis, coculture experiments, or adoptive transfer.

Columns

For positive selection: MS, LS, XS, or autoMACS
®
Columns. For depletion: LD, D, or autoMACS Columns.

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CD25 MicroBead Kit, mouse

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