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Isolation of CD1c (BDCA-1 )+ dendritic cells from rhesus monkey PBMCs using the CD1c (BDCA-1) + Dendritic Cell Isolation Kit. Cells were incubated with CD1c (BDCA-1)-PE and subsequently magnetically labeled with CD20 MicroBeads. B cells were depleted using an LD Column. The B cell depleted fraction was magnetically labeled with Anti-PE MicroBeads and separated over an MS Column. Cell samples were stained with CD20-FITC and CD14-APC. Cell debris and dead cells were excluded from the analysis based on scatter signals and PI fluorescence. |
PBMCs before separation | B cell–depleted flow-through fraction |
CD1c (BDCA-1) + Dendritic Cell Isolation Kit | CD1c (BDCA-1) + Dendritic Cell Isolation Kit |
Counter-staining of B cell depleted flow-through fraction with CD14-APC | CD1c (BDCA-1) + blood dendritic cells after enrichment |
CD1c (BDCA-1) + Dendritic Cell Isolation Kit | CD1c (BDCA-1) + Dendritic Cell Isolation Kit |
Isolation of CD1c (BDCA-1 )+ dendritic cells from rhesus monkey PBMCs using the CD1c (BDCA-1) + Dendritic Cell Isolation Kit. Cells were incubated with CD1c (BDCA-1)-PE and subsequently magnetically labeled with CD20 MicroBeads. B cells were depleted using an LD Column. The B cell depleted fraction was magnetically labeled with Anti-PE MicroBeads and separated over an MS Column. Cell samples were stained with CD20-FITC and CD14-APC. Cell debris and dead cells were excluded from the analysis based on scatter signals and PI fluorescence. |
PBMCs before separation | B cell–depleted flow-through fraction |
CD1c (BDCA-1) + Dendritic Cell Isolation Kit | CD1c (BDCA-1) + Dendritic Cell Isolation Kit |
Counter-staining of B cell depleted flow-through fraction with CD14-APC | CD1c (BDCA-1) + blood dendritic cells after enrichment |
CD1c (BDCA-1) + Dendritic Cell Isolation Kit | CD1c (BDCA-1) + Dendritic Cell Isolation Kit |
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