CD19 CAR⁺ T cells were stained with CD19 CAR Detection Reagent (anti-FMC63), as well as with CD3 antibodies. Cells were analyzed by flow cytometry using the MACSQuant^® Analyzer. Cells were pregated on CD3⁺ cells. Cell debris and dead cells were excluded from the analysis based on scatter signals and 7-AAD fluorescence.

Human primary T cells expressing a FMC63-based CD19 CAR were mixed with murine T cells expressing a Glypican3 CAR in a 1:1 ratio and spun onto a microscope slide. Cells were fixed with 4% PFA and subjected to MACSima™ Imaging Cyclic Staining (MICS) analysis using the MACSima Imaging System. The image shows stainings of CD19 CAR FMC63 Idiotype Antibody-PE (REA1297, magenta), mouse CD45-FITC (REA737, red) and human CD45-PE (REA747, turquoise). The concurrent staining of CD19 CAR and human CD45, but not mouse CD45, demonstrates specificity of REA1297 for the CD19 CAR construct. Image courtesy of Dr. Christian Seitz, University Children's Hospital Tübingen.