Flow cytometric comparison of different clones for Biotin. Human peripheral blood mononuclear cells (PBMCs) loaded with CD4-Biotin were stained with Biotin antibodies and with a suitable counterstaining. As a control, unloaded human peripheral blood mononuclear cells (PBMCs) were stained with the antibody Biotin and an appropriate counterstain. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Flow cytometric comparison of different clones for Biotin. Human peripheral blood mononuclear cells (PBMCs) loaded with CD4-Biotin were stained with Biotin antibodies and with a suitable counterstaining. As a control, unloaded human peripheral blood mononuclear cells (PBMCs) were stained with the antibody Biotin and an appropriate counterstain. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Flow cytometric comparison of different clones for Biotin. Human peripheral blood mononuclear cells (PBMCs) loaded with CD4-Biotin were stained with Biotin antibodies and with a suitable counterstaining. As a control, unloaded human peripheral blood mononuclear cells (PBMCs) were stained with the antibody Biotin and an appropriate counterstain. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Flow cytometric comparison of different clones for Biotin. Human peripheral blood mononuclear cells (PBMCs) loaded with CD4-Biotin were stained with Biotin antibodies and with a suitable counterstaining. As a control, unloaded human peripheral blood mononuclear cells (PBMCs) were stained with the antibody Biotin and an appropriate counterstain. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Flow cytometric comparison of different clones for Biotin. Human peripheral blood mononuclear cells (PBMCs) loaded with CD4-Biotin were stained with Biotin antibodies and with a suitable counterstaining. As a control, unloaded human peripheral blood mononuclear cells (PBMCs) were stained with the antibody Biotin and an appropriate counterstain. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Flow cytometric comparison of different clones for Biotin. Human peripheral blood mononuclear cells (PBMCs) loaded with CD4-Biotin were stained with Biotin antibodies and with a suitable counterstaining. As a control, unloaded human peripheral blood mononuclear cells (PBMCs) were stained with the antibody Biotin and an appropriate counterstain. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Flow cytometric comparison of different clones for Biotin. Human peripheral blood mononuclear cells (PBMCs) loaded with CD4-Biotin were stained with Biotin antibodies and with a suitable counterstaining. As a control, unloaded human peripheral blood mononuclear cells (PBMCs) were stained with the antibody Biotin and an appropriate counterstain. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Comparison of staining pattern on non-fixed and fixed cells using Biotin (REA746). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Comparison of staining pattern on non-fixed and fixed cells using Biotin (REA746). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Comparison of staining pattern on non-fixed and fixed cells using Biotin (REA746). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Comparison of staining pattern on non-fixed and fixed cells using Biotin (REA746). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.