The CD141 (BDCA-3) MicroBead Kit has been developed for the isolation of CD141 (BDCA-3)
++
cells (namely type-2 MDCs or MDC2) from PBMCs. CD141 (BDCA-3)
1
is expressed at high levels only on a minor subpopulation of myeloid dendritic cells (MDCs) in human blood (about 0.04 % of all PBMCs).

Data and images for CD141 (BDCA-3) MicroBead Kit, human

Figures

Figure 1

Isolation of CD141 (BDCA-3)
++
myeloid dendritic cells from PBMCs using the CD141 (BDCA-3) MicroBead Kit, a MiniMACS™ Separator, and MS Columns. Cells were stained with CD141 (BDCA-3)-PE and CD14-FITC. Only very few CD1c (BDCA-1)
+
myeloid dendritic cells, CD303 (BDCA-2)
+
plasmacytoid dendritic cells, and CD14
+
monocytes are coenriched, although they express CD141 (BDCA-3) at low levels.
PBMCs before separation
Enriched CD141 (BDCA-3)
++
myeloid dendritic cells
View details

Figure 1

Isolation of CD141 (BDCA-3)
++
myeloid dendritic cells from PBMCs using the CD141 (BDCA-3) MicroBead Kit, a MiniMACS™ Separator, and MS Columns. Cells were stained with CD141 (BDCA-3)-PE and CD14-FITC. Only very few CD1c (BDCA-1)
+
myeloid dendritic cells, CD303 (BDCA-2)
+
plasmacytoid dendritic cells, and CD14
+
monocytes are coenriched, although they express CD141 (BDCA-3) at low levels.
View details

Figure 1

Isolation of CD141 (BDCA-3)
++
myeloid dendritic cells from PBMCs using the CD141 (BDCA-3) MicroBead Kit, a MiniMACS™ Separator, and MS Columns. Cells were stained with CD141 (BDCA-3)-PE and CD14-FITC. Only very few CD1c (BDCA-1)
+
myeloid dendritic cells, CD303 (BDCA-2)
+
plasmacytoid dendritic cells, and CD14
+
monocytes are coenriched, although they express CD141 (BDCA-3) at low levels.

Figure 2

View details
CD141 (BDCA-3)
++
type-2 myeloid dendritic cells, May-Grünwald/Giemsa stained.

Figure 2

CD141 (BDCA-3)
++
type-2 myeloid dendritic cells, May-Grünwald/Giemsa stained.

Specifications for CD141 (BDCA-3) MicroBead Kit, human

Overview

The CD141 (BDCA-3) MicroBead Kit has been developed for the isolation of CD141 (BDCA-3)
++
cells (namely type-2 MDCs or MDC2) from PBMCs. CD141 (BDCA-3)
1
is expressed at high levels only on a minor subpopulation of myeloid dendritic cells (MDCs) in human blood (about 0.04 % of all PBMCs).

Detailed product information

Background information

CD141 (BDCA-3)
++
MDCs are CD11c
dim
, CD123
, CD1c (BDCA-1)
, CD4
+
, and do not express lineage markers such as CD3, CD14, CD16, CD19, CD20, and CD56. They express myeloid markers such as CD13 and CD33 and are monocytoid in appearance (fig. 2). In contrast to CD1c (BDCA-1)
+
MDCs, CD141 (BDCA-3)
++
MDCs do not express CD2 and Fc receptors such as CD32, CD64, and FceRI. Furthermore, CD141 (BDCA-3)
++
MDCs differ from CD1c (BDCA-1)
+
MDCs in terms of Toll-like receptor expression, cytokine production, and T helper cell polarization.
3
In order to discriminate the CD141 (BDCA-3)
++
from CD1c (BDCA-1)
+
MDCs, they have been designated type-2 MDCs (MDC2s).
The CD141 (BDCA-3) antigen is also expressed at a much lower level on CD1c (BDCA-1)
+
MDCs, plasmacytoid dendritic cells, monocytes, and granulocytes in blood.

Downstream applications

CD141 (BDCA-3)
++
MDCs were separated for their further phenotypic and functional characterization
1,3
, for gene clustering
6
, or for expression analyses of newly defined antigen EMR2 on different dendritic cell subsets
4
. The CD141 (BDCA-3) MicroBead Kit was also used for isolation of human CD141 (BDCA-3)
++
MDCs from mice engrafted with CD34
+
hematopoietic progenitors.
5

Columns

MS, LS, or autoMACS
®
Columns.

Resources for CD141 (BDCA-3) MicroBead Kit, human

References for CD141 (BDCA-3) MicroBead Kit, human

Publications

  1. Dzionek, A. et al. (2000) BDCA-2, BDCA-3, BDCA-4: Three markers for distinct subsets of dendritic cells in human peripheral blood. J. Immunol. 165: 6037-6046
  2. Grabbe, S. et al. (2000) Dendritic cells: multi-lineal and multi-functional. Immunol. Today 21: 431-433
  3. Lebre, C. M. et al. (2003)
    BDCA-3
    hi
    dendritic cells: a novel subset with distinct phenotypical characteristics. Doctoral dissertation.
    University of Amsterdam, Netherlands
  4. Kwakkenbos, M. J. et al. (2002) The human EGF-TM7 family member EMR2 is a heterodimeric receptor expressed on myeloid cells. J. Leukoc. Biol. 71(5): 854-862
  5. Palucka, A. K. et al. (2003)
    Human dendritic cell subsets in NOD/SCID mice engrafted with CD34
    +
    hematopoietic progenitors.
    Blood 102: 3302-3310
  6. Lindstedt, M. et al. (2005)
    Gene family clustering identifies functionally associated subsets of human
    in vivo
    blood and tonsillar dendritic cells.
    J. Immunol. 175: 4839-4846

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