In mice CD11c is a well-established, yet not exclusive, pan-DC marker. CD11c MicroBeads UltraPure have been optimized for the isolation of mouse DCs from single-cell suspensions from lymphoid and non-lymphoid tissues. The CD11c MicroBeads UltraPure allow for the rapid and simple isolation of all defined murine DC subsets. The UltraPure beads greatly improve recovery and purity of the sorted population by specifically enriching viable cells.

Data and images for CD11c MicroBeads UltraPure, mouse

Figures

Figure 1

CD11c
+
DCs were isolated from mouse spleen using CD11c MicroBeads UltraPure, two MS Columns, and a MiniMACS™ Separator. Cells were fluorescently stained with CD11c-VioBlue (# 130‑102‑413) and analyzed by flow cytometry using the MACSQuant
®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
Before separation
CD11c
+
dendritic cells
View details

Figure 1

CD11c
+
DCs were isolated from mouse spleen using CD11c MicroBeads UltraPure, two MS Columns, and a MiniMACS™ Separator. Cells were fluorescently stained with CD11c-VioBlue (# 130‑102‑413) and analyzed by flow cytometry using the MACSQuant
®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
View details

Figure 1

CD11c
+
DCs were isolated from mouse spleen using CD11c MicroBeads UltraPure, two MS Columns, and a MiniMACS™ Separator. Cells were fluorescently stained with CD11c-VioBlue (# 130‑102‑413) and analyzed by flow cytometry using the MACSQuant
®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Specifications for CD11c MicroBeads UltraPure, mouse

Overview

In mice CD11c is a well-established, yet not exclusive, pan-DC marker. CD11c MicroBeads UltraPure have been optimized for the isolation of mouse DCs from single-cell suspensions from lymphoid and non-lymphoid tissues. The CD11c MicroBeads UltraPure allow for the rapid and simple isolation of all defined murine DC subsets. The UltraPure beads greatly improve recovery and purity of the sorted population by specifically enriching viable cells.

Detailed product information

Background information

The mouse CD11c antigen is present in dendritic cells in lymphoid organs and blood, in Langerhans cells in the epidermis, in dendritic cell progenitors in the bone marrow, and in in vitro generated bone marrow–derived dendritic cells. In mouse spleen and lymph node, CD11c is expressed at high levels on conventional CD11c
+
CD45R
mPDCA-1
dendritic cells, and at moderate levels on CD11c
+
CD45R
+
mPDCA-1
+
plasmacytoid dendritic cells. CD11c is reported to be weakly expressed on NK cells, B cells, and T cell subsets. About 1–3% of splenocytes, 2% of bone marrow cells, as well as <1% of lymph node cells and thymocytes express CD11c.

Downstream applications

DCs isolated with CD11c MicroBeads have been used for phenotypical and functional analyses, e.g. T cell activation, polarization, and tolerance induction in different experimental murine models. CD11c MicroBeads were used to isolate highly pure DCs from spleen and lymph nodes
1,2
, Peyer's patches
3
, colonic lamina propria
4
, bone marrow
5
, epidermis
6
, lung
7
, liver
8
, or brain
9

Columns

For positive selection: MS, LS, XS, or autoMACS
®
Columns. For depletion: LD, D, or autoMACS Columns.

References for CD11c MicroBeads UltraPure, mouse

Publications

  1. Williamson, E. et al. (2002) Regulation of mucosal dendritic cell function by receptor activator of NF-κB (Rank)/Rank ligand interactions: impact on tolerance induction. J. Immunol. 169: 3606-3612
  2. Ing, R. et al. (2006) Interaction of mouse dendritic cells and malaria-infected erythrocytes: uptake, maturation, and antigen presentation. J. Immunol. 176: 441-450
  3. Kadaoui. K. A. and Corthésy, B. et al. (2004) Isolation of dendritic cells from mouse Peyer’s patches using magnetic cell sorting. MACS&more 8(1): 10-12
  4. Krajina, T. et al. (2003)
    Colonic lamina propria dendritic cells in mice with CD4
    +
    T cell-induced colitis.
    Eur. J. Immunol. 33: 1073-1083
  5. Hou, W. S. and van Parijs, L. et al. (2004) A Bcl-2-dependent molecular timer regulates the lifespan and immunogenicity of dendritic cells. Nat. Immunol. 5: 583-589
  6. Sato, T. et al. (2002) Consequences of OX40-OX40 ligand interactions in langerhans cell function: enhanced contact hypersensitivity responses in OX40L-transgenic mice. Eur. J. Immunol. 32: 3326-3335
  7. Swanson, K. A. et al. (2004) Flt3-Ligand, Il-4, GM-CSF, and adherencemediated isolation of murine lung dendritic cells: assessment of isolation technique on phenotype and function. J. Immunol. 173: 4875-4881
  8. Johansson, C. and Wick, M. J. (2004)
    Liver dendritic cells present bacterial antigens and produce cytokines upon
    Salmonella
    encounter.
    J. Immunol. 172: 2496-2503
  9. Fischer, H. G. et al. (2000)
    Phenotype and functions of brain dendritic cells emerging during chronic infection of mice with
    Toxoplasma gondii
    .
    J. Immunol. 164: 4826-4834
Video

How to isolate CD11c+ dendritic cells from mouse spleen

Watch how easy it is to manually separate DCs from mouse tissue using MACS Technology.

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