Applications:
FC

Specifications for BM-MSC Analysis Cocktail Kit, anti-human

Overview

The BM-MSC Analysis Cocktail Kit, anti-human has been designed for the standardized analysis and quantification of mesenchymal stem cells (MSCs) from human bone marrow (BM) samples based on the expression of CD271 (LNGFR). The kit includes a ready-to-use analysis and control cocktail for fast and robust enumeration of MSCs. The additional MSC marker MSCA-1 allows the identification of MSCs with highly proliferative potential.

Detailed product information

Background information

BM-MSC Analysis Cocktail, anti-human containing:
  • CD45 Antibody, anti-human, FITC (clone: 5B1)
  • CD235a (Glycophorin A) Antibody, anti-human, VioBlue®, REAfinity™ (clone: REA175)
  • CD271 (LNGFR) Antibody, anti-human, PE (clone: ME20.4-1.H4)
  • MSCA-1 Antibody, anti-human, APC (clone: W8B2)
BM-MSC Control Cocktail, anti-human containing:
  • Isotype Control Antibody, mouse IgG1, APC (clone: IS5-21F5)
  • CD45 Antibody, anti-human, FITC (clone: 5B1)
  • CD235a (Glycophorin A) Antibody, anti-human, VioBlue®, REAfinity (clone: REA175)
  • CD271 (LNGFR) Antibody, anti-human, PE (clone: ME20.4-1.H4)
Additionally
  • Red Blood Cell Lysis Solution (10×)
  • FcR Blocking Reagent, human
  • 7-AAD Staining Solution
I
nstrument details
Flow cytometer equipped with a red (640 nm), a blue (488 nm), and a violet (405 nm) laser.

Applications

Analysis and standardized quantification of human MSCs from fresh bone marrow samples by flow cytometry.

Resources for BM-MSC Analysis Cocktail Kit, anti-human

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for BM-MSC Analysis Cocktail Kit, anti-human

Publications

  1. Cuthbert, R. et al. (2012) Single-platform quality control assay to quantify multipotential stromal cells in bone marrow aspirates prior to bulk manufacture or direct therapeutic use. Cytotherapy 14(4): 431-440

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