The Spleen Dissociation Kit has been developed for the gentle and rapid generation of single-cell suspensions from mouse spleen. It is optimized for high yield of leukocytes, especially dendritic cells, while preserving all cell surface epitopes. The kit is ideally suited for the time-saving and reproducible preparation of single-cell suspensions in combination with the gentleMACS™ Dissociators.

Data and images for Spleen Dissociation Kit, mouse

Figures

Figure 1

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Mouse spleen single-cell suspensions were stained with CD11c-APC and Anti-mPDCA-1-FITC and analyzed using the MACSQuant
®
Analyzer. Viable leukocytes are shown.

Figure 1

Mouse spleen single-cell suspensions were stained with CD11c-APC and Anti-mPDCA-1-FITC and analyzed using the MACSQuant
®
Analyzer. Viable leukocytes are shown.

Figure 2

View details
Mouse spleen single-cell suspensions were stained with CD8a-FITC and CD4-PE and analyzed using the MACSQuant
®
Analyzer. Viable leukocytes are shown.

Figure 2

Mouse spleen single-cell suspensions were stained with CD8a-FITC and CD4-PE and analyzed using the MACSQuant
®
Analyzer. Viable leukocytes are shown.

Specifications for Spleen Dissociation Kit, mouse

Overview

The Spleen Dissociation Kit has been developed for the gentle and rapid generation of single-cell suspensions from mouse spleen. It is optimized for high yield of leukocytes, especially dendritic cells, while preserving all cell surface epitopes. The kit is ideally suited for the time-saving and reproducible preparation of single-cell suspensions in combination with the gentleMACS™ Dissociators.

Detailed product information

Applications

The Spleen Dissociation Kit is recommended for the generation of single-cell suspensions from mouse spleen prior to the isolation of dendritic cells, for example, when using CD11c MicroBeads, Anti-mPDCA-1 MicroBeads, or Pan DC MicroBeads.

Resources for Spleen Dissociation Kit, mouse

Video

How to dissociate mouse spleen tissue with the gentleMACS Dissociator

Watch how simple it is to dissociate mouse spleen tissue with the gentleMACS Dissociator.

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Spleen Dissociation Kit, mouse

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