MACSPlex Kits | Multiplex assays | Kits and support reagents | MACS Flow Cytometry | Products | Miltenyi Biotec

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Overview of available MACSPlex Kits

Application category	Kit name	Number of analytes	Target analytes	MACSQuantify Software version	Express Mode package
Human cytokines	MACSPlex Cytokine 12 Kit, human	12	IL-2, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12, IL-17A, GM-CSF, IFN-α, IFN-γ, TNF-α	2.5 and higher	25.2.9990 and higher
Mouse cytokines	MACSPlex Cytokine 10 Kit, mouse	10	IL-2, IL-4, IL-5, IL-10, IL-12, IL-17A, IL-23, GM-CSF, IFN-γ, TNF-α	2.5 and higher	25.3.10934 and higher
Human cytotoxicity	MACSPlex Cytotoxic T/NK Cell Kit, human	12	IL-2, IL-4, IL-6, IL-10, IL17A, IL-21, GM-CSF, Granzyme B, IFN-γ, MCP-1, TNF-α, Perforin	2.13.1 and higher	213.2.20284 and higher*
Human exosomes	MACSPlex Exosome Kit, human	37 exosome markers and 2 controls	CD3, CD4, CD19, CD8, HLA-DRDPDQ, CD56, CD105, CD2, CD1c, CD25, CD49e, ROR1, CD209, CD9, SSEA-4, HLA-ABC, CD63, CD40, CD62P, CD11c, CD81, MCSP, CD146, CD41b, CD42a, CD24, CD86, CD44, CD326, CD133/1, CD29, CD69, CD142, CD45, CD31, CD20, CD14REA Control, mIgG1 control	2.8 and higher	28.1.15443 and higher
Human miRNA	MACSPlex miRNA Kit - Cancer, human	35 miRNAs and 4 controls	miR-378b, miR-141-3p, miR-23a-3p, miR-17-5p, miR-20a-5p, miR-200a-3p, miR-29a-3p, miR-429, miR-143-3p, miR-21-5p, miR-19b-3p, miR-31-5p, let-7f-5p, miR-335-5p, miR-200b-3p, miR-34a-5p, let-7a-5p, miR-101-3p, miR-373-3p, miR-122-5p, miR-107, hsa.miR-30b-5p, miR-15b-5p, miR-520c-3p, miR-200c-3p, miR-9-5p, miR-23b-3p, let-7g-5p, miR-145-5p, let-7b-5p, miR-103a-3p, miR-10b-5p, miR-10b-5p, miR-16-5p, miR-126-3p, miR-15a-5pControl-4-Neg, Control-1-U6, Control-2-5.8S, Control-3-C4	2.6 and higher	26.1.12747 and higher

*Note: to get the new Express Mode package please contact your local support team

Basic principle

Principle of MACSPlex T/NK Cell Assays

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Samples containing unknown levels of analytes are incubated with the antibody-coated MACSPlex Capture Beads, and analytes bind to the specific antibody. Two detection reagents, composed of a cocktail of either biotin-conjugated or APC-conjugated antibodies specific for the analytes, are added. Consequently, sandwich complexes are formed between the MACSPlex Capture Beads, the analyte, and the Detection Reagent Biotin while the Detection Reagent APC binds to the biotinylated detection antibodies. These complexes can be analyzed based on the fluorescence characteristics.

Standards of known quantities of given analytes are provided with the kit and are used for the quantification of the analytes within the unknown samples.

Prerequisites

For MACSPlex assays a flow cytometer with a blue (e.g. 488 nm) and a red (e.g. 635 nm) laser with the capability to detect FITC, PE, and APC is necessary.

Analysis with MACSQuant Analyzers and Express Modes

Exemplary analysis and results using a MACSPlex Cytokine Kit

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When using the MACSQuant Analyzer 10 or MACSQuant X, our MACSplex Kits can be combined with fully automated Express Modes.

By this, an automated gating strategy to populations of interest will be applied and the gates will be automatically adjusted for each data file individually to achieve optimal results.

Furthermore, a one-click batch analysis of all samples belonging to one experiment can be performed to directly compare your data. The concentrations of each analyte will be presented as an exportale table as well as a ready-to-publish graph.

ExpressModes are compatible with the MACSQuant Analyzer 10 and MACSQuant X instruments and with the MACSQuantify™ Software already installed on your MACSQuant Analyzer. For the required software version, please refer to the table above.

Analysis with other instruments

For all users running MACSPlex assays on a different flow cytometer, subsequent analysis can be performed on your computer. For this, you can use our Flowlogic™ Software in combination with the Beadlogic™ Add-on specifically developed to analyze multiplex assays.

30-day trial versions are available for both Flowlogic Software and the Beadlogic Add-on.

Additionally, third-party software can be used for analysis.