Immunophenotyping of T cells from mouse spleen using flow cytometry

Miltenyi Biotec-tested panel 11 (MBTP 11)

This application protocol describes the flow cytometric analysis of major T cell subsets after spleen dissociation from healthy C57BL/6 mice. Mouse spleens can easily be dissociated using the gentleMACS™ Dissociators to quickly obtain viable single cell suspensions ready for downstream applications, such as flow cytometric analysis.

Protocol

Materials

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