Immunophenotyping of regulatory 
T cells from mouse spleen using flow cytometry

Miltenyi Biotec-tested panel 19 (MBTP 19)

This application protocol describes the flow cytometric analysis of regulatory T cells (Tregs) after spleen dissociation from healthy C57BL/6 mice. Mouse spleens are easily dissociated using the gentleMACS™ Dissociators to quickly obtain viable single cell suspensions ready for flow cytometric analysis.

Protocol

Materials

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