The Pan Monocyte Isolation Kit is an indirect magnetic labeling system for the isolation of untouched monocytes from human PBMCs. This Kit allows the simultaneous enrichment of classical (CD14
++
CD16
), non classical (CD14
+
CD16
++
) and intermediate (CD14
++
CD16
+
) monocytes.
1
Highly pure unlabeled monocytes are obtained by depletion of the magnetically labeled cells.

Data and images for Pan Monocyte Isolation Kit, human

Figures

Figure 1

Isolation of untouched monocytes from PBMCs by using the Pan Monocyte Isolation Kit and an LS Column. Cells were fluorescently stained with CD14-FITC, CD16-PE, and Anti-Slan (M-DC8)-APC (# 130-093-031) and analyzed using the MACSQuant® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and PI fluorescence.
PBMCs before separation
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Figure 1

Isolation of untouched monocytes from PBMCs by using the Pan Monocyte Isolation Kit and an LS Column. Cells were fluorescently stained with CD14-FITC, CD16-PE, and Anti-Slan (M-DC8)-APC (# 130-093-031) and analyzed using the MACSQuant® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and PI fluorescence.
View details

Figure 1

Isolation of untouched monocytes from PBMCs by using the Pan Monocyte Isolation Kit and an LS Column. Cells were fluorescently stained with CD14-FITC, CD16-PE, and Anti-Slan (M-DC8)-APC (# 130-093-031) and analyzed using the MACSQuant® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and PI fluorescence.
Isolated monocytes
View details

Figure 1

Isolation of untouched monocytes from PBMCs by using the Pan Monocyte Isolation Kit and an LS Column. Cells were fluorescently stained with CD14-FITC, CD16-PE, and Anti-Slan (M-DC8)-APC (# 130-093-031) and analyzed using the MACSQuant® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and PI fluorescence.
View details

Figure 1

Isolation of untouched monocytes from PBMCs by using the Pan Monocyte Isolation Kit and an LS Column. Cells were fluorescently stained with CD14-FITC, CD16-PE, and Anti-Slan (M-DC8)-APC (# 130-093-031) and analyzed using the MACSQuant® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and PI fluorescence.

Specifications for Pan Monocyte Isolation Kit, human

Overview

The Pan Monocyte Isolation Kit is an indirect magnetic labeling system for the isolation of untouched monocytes from human PBMCs. This Kit allows the simultaneous enrichment of classical (CD14
++
CD16
), non classical (CD14
+
CD16
++
) and intermediate (CD14
++
CD16
+
) monocytes.
1
Highly pure unlabeled monocytes are obtained by depletion of the magnetically labeled cells.

Detailed product information

Detailed separation procedure

Non-monocytes, such as T cells, NK cells, B cells, dendritic cells, and basophils are indirectly magnetically labeled using a cocktail of biotin-conjugated antibodies and Anti-Biotin MicroBeads.

Downstream applications

  • Functional studies on monocytes, in which any effect due to antibody binding to target cells should be avoided.
  • Studies on monocyte activation, differentiation, cytokine secretion etc.
  • Studies on signal transduction in monocytes.
  • Studies on antigen uptake (e.g. phagocytosis) and antigen presentation by monocytes.
  • In vitro differentiation of blood monocytes into dendritic cells.

Columns

MS, LS, XS, or autoMACS
®
Columns.

References for Pan Monocyte Isolation Kit, human

Publications

  1. Ziegler-Heitbrock et al. (2010) Nomenclature of monocytes and dendritic cells in blood. Blood 116: e74-80

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