CD14 MicroBeads can be used for the positive selection or depletion of human monocytes and macrophages from cord blood or PBMCs, as well as pleural, peritoneal, or synovial fluids or from various tissues, such as spleen and lymph node.
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Monocytes were isolated from PBMCs using CD14 MicroBeads and subsequently cultured for 7 days with IL-4 and GM-CSF to generate immature Mo-DCs and were, finally, cultured for additional 3 days with TNF-α for maturation. Mature Mo-DCs were stained for expression of CD1a, costimulatory molecules (CD80, CD86), CD83 and HLA-DR. | |
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 View details CD14 MicroBeads, humanFigure 2Monocytes were isolated from PBMCs using CD14 MicroBeads and subsequently cultured for 7 days with IL-4 and GM-CSF to generate immature Mo-DCs and were, finally, cultured for additional 3 days with TNF-α for maturation. Mature Mo-DCs were stained for expression of CD1a, costimulatory molecules (CD80, CD86), CD83 and HLA-DR. |  View details CD14 MicroBeads, humanFigure 2Monocytes were isolated from PBMCs using CD14 MicroBeads and subsequently cultured for 7 days with IL-4 and GM-CSF to generate immature Mo-DCs and were, finally, cultured for additional 3 days with TNF-α for maturation. Mature Mo-DCs were stained for expression of CD1a, costimulatory molecules (CD80, CD86), CD83 and HLA-DR. |
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 View details CD14 MicroBeads, humanFigure 2Monocytes were isolated from PBMCs using CD14 MicroBeads and subsequently cultured for 7 days with IL-4 and GM-CSF to generate immature Mo-DCs and were, finally, cultured for additional 3 days with TNF-α for maturation. Mature Mo-DCs were stained for expression of CD1a, costimulatory molecules (CD80, CD86), CD83 and HLA-DR. |  View details CD14 MicroBeads, humanFigure 2Monocytes were isolated from PBMCs using CD14 MicroBeads and subsequently cultured for 7 days with IL-4 and GM-CSF to generate immature Mo-DCs and were, finally, cultured for additional 3 days with TNF-α for maturation. Mature Mo-DCs were stained for expression of CD1a, costimulatory molecules (CD80, CD86), CD83 and HLA-DR. |
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 View details CD14 MicroBeads, humanFigure 2Monocytes were isolated from PBMCs using CD14 MicroBeads and subsequently cultured for 7 days with IL-4 and GM-CSF to generate immature Mo-DCs and were, finally, cultured for additional 3 days with TNF-α for maturation. Mature Mo-DCs were stained for expression of CD1a, costimulatory molecules (CD80, CD86), CD83 and HLA-DR. |
With this product we achieved successful isolation of monocytes from human PBMCs with >90% purity of CD14+ monocytes and functionally active cells (phagocytosis and cytokine release)
Our lab requires highly pure immune cell subsets, such as monocytes and macrophages, for our experiments. To purify monocytes and macrophages from human peripheral blood mononuclear cells (PBMC), we regularly employ Miltenyi anti-human CD14 microbeads for magnetic positive selection. This product was initially chosen because of the minimal optimization and straightforwardness of the protocol.
Our laboratory investigates different ways to improve immune responses against HIV, including enhancement of the capacity of effector cells to kill HIV-infected cells. We are currently investigating the capacity of an engineered antibody to trigger ADCC activity in NK cells and monocytes in in vitro assays against an HIV-infected cell line. We use CD14 beads for purification of monocytes and macrophages.
Monocytes were isolated from PBMCs using CD14 MicroBeads and subsequently cultured for 7 days with IL-4 and GM-CSF to generate immature Mo-DCs and were, finally, cultured for additional 3 days with TNF-α for maturation. Mature Mo-DCs were stained for expression of CD1a, costimulatory molecules (CD80, CD86), CD83 and HLA-DR. | |
A: | B: |
View details CD14 MicroBeads, humanFigure 2Monocytes were isolated from PBMCs using CD14 MicroBeads and subsequently cultured for 7 days with IL-4 and GM-CSF to generate immature Mo-DCs and were, finally, cultured for additional 3 days with TNF-α for maturation. Mature Mo-DCs were stained for expression of CD1a, costimulatory molecules (CD80, CD86), CD83 and HLA-DR. | View details CD14 MicroBeads, humanFigure 2Monocytes were isolated from PBMCs using CD14 MicroBeads and subsequently cultured for 7 days with IL-4 and GM-CSF to generate immature Mo-DCs and were, finally, cultured for additional 3 days with TNF-α for maturation. Mature Mo-DCs were stained for expression of CD1a, costimulatory molecules (CD80, CD86), CD83 and HLA-DR. |
C: | D: |
View details CD14 MicroBeads, humanFigure 2Monocytes were isolated from PBMCs using CD14 MicroBeads and subsequently cultured for 7 days with IL-4 and GM-CSF to generate immature Mo-DCs and were, finally, cultured for additional 3 days with TNF-α for maturation. Mature Mo-DCs were stained for expression of CD1a, costimulatory molecules (CD80, CD86), CD83 and HLA-DR. | View details CD14 MicroBeads, humanFigure 2Monocytes were isolated from PBMCs using CD14 MicroBeads and subsequently cultured for 7 days with IL-4 and GM-CSF to generate immature Mo-DCs and were, finally, cultured for additional 3 days with TNF-α for maturation. Mature Mo-DCs were stained for expression of CD1a, costimulatory molecules (CD80, CD86), CD83 and HLA-DR. |
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View details CD14 MicroBeads, humanFigure 2Monocytes were isolated from PBMCs using CD14 MicroBeads and subsequently cultured for 7 days with IL-4 and GM-CSF to generate immature Mo-DCs and were, finally, cultured for additional 3 days with TNF-α for maturation. Mature Mo-DCs were stained for expression of CD1a, costimulatory molecules (CD80, CD86), CD83 and HLA-DR. |
With this product we achieved successful isolation of monocytes from human PBMCs with >90% purity of CD14+ monocytes and functionally active cells (phagocytosis and cytokine release)
Our lab requires highly pure immune cell subsets, such as monocytes and macrophages, for our experiments. To purify monocytes and macrophages from human peripheral blood mononuclear cells (PBMC), we regularly employ Miltenyi anti-human CD14 microbeads for magnetic positive selection. This product was initially chosen because of the minimal optimization and straightforwardness of the protocol.
Our laboratory investigates different ways to improve immune responses against HIV, including enhancement of the capacity of effector cells to kill HIV-infected cells. We are currently investigating the capacity of an engineered antibody to trigger ADCC activity in NK cells and monocytes in in vitro assays against an HIV-infected cell line. We use CD14 beads for purification of monocytes and macrophages.
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