Neonatal Cardiac Endothelial Cell Isolation Kit, mouse

Neonatal Cardiac Endothelial Cell Isolation Kit, mouse

The Neonatal Cardiac Endothelial Cell Isolation Kit, mouse has been developed for the two-step isolation of vital murine cardiac endothelial cells (P0-P3). Enriched cardiac endothelial cells are fully functional and can be used for various downstream applications.

Data and images
Specifications

Product data and images for Neonatal Cardiac Endothelial Cell Isolation Kit, mouse

Figures

Figure 1

Cardiac endothelial cells were isolated from P1 CD-1 mouse hearts by using the Neonatal Cardiac Endothelial Cell Isolation Kit, one LD Column with MidiMACS

^™

Separator, and one MS Column with MiniMACS™ Separator. The cells were fluorescently stained with CD90.2-APC (# 130-091-790) and CD31-PE (# 130-097-422) and analyzed by flow cytometry using the MACSQuant

^®

Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Figure 1

Cardiac endothelial cells were isolated from P1 CD-1 mouse hearts by using the Neonatal Cardiac Endothelial Cell Isolation Kit, one LD Column with MidiMACS

^™

Separator, and one MS Column with MiniMACS™ Separator. The cells were fluorescently stained with CD90.2-APC (# 130-091-790) and CD31-PE (# 130-097-422) and analyzed by flow cytometry using the MACSQuant

^®

Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Figure 1

Cardiac endothelial cells were isolated from P1 CD-1 mouse hearts by using the Neonatal Cardiac Endothelial Cell Isolation Kit, one LD Column with MidiMACS

^™

Separator, and one MS Column with MiniMACS™ Separator. The cells were fluorescently stained with CD90.2-APC (# 130-091-790) and CD31-PE (# 130-097-422) and analyzed by flow cytometry using the MACSQuant

^®

Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Specifications for Neonatal Cardiac Endothelial Cell Isolation Kit, mouse

Overview

The Neonatal Cardiac Endothelial Cell Isolation Kit, mouse has been developed for the two-step isolation of vital murine cardiac endothelial cells (P0-P3). Enriched cardiac endothelial cells are fully functional and can be used for various downstream applications.

Detailed product information

Background information

Besides cardiomyocytes and fibroblasts, endothelial cells represent the third most frequent cell population in the neonatal mouse heart. Endothelial cells form the inner layer of blood vessels and play a key role in vascular development, homeostasis and remodeling. The Neonatal Cardiac Endothelial Cell Isolation Kit, mouse has been designed for enrichment of cardiac endothelial cells from dissociated neonatal mouse hearts. The isolation of cardiac endothelial cells has successfully been tested using neonatal mouse hearts from postnatal day 0 to day 3 (P0-P3). For optimal use, the Neonatal Cardiac Endothelial Cell Isolation Kit should be used in combination with the Neonatal Heart Dissociation Kit, mouse and rat (# 130-098-373).

Columns

For the first magnetic separation (depletion): LD or autoMACS

^®

Columns. For the second magnetic separation (positive selection): MS or autoMACS Columns.

Data and images

Data and images for Neonatal Cardiac Endothelial Cell Isolation Kit, mouse

Figures

Figure 1

Cardiac endothelial cells were isolated from P1 CD-1 mouse hearts by using the Neonatal Cardiac Endothelial Cell Isolation Kit, one LD Column with MidiMACS

^™

Separator, and one MS Column with MiniMACS™ Separator. The cells were fluorescently stained with CD90.2-APC (# 130-091-790) and CD31-PE (# 130-097-422) and analyzed by flow cytometry using the MACSQuant

^®

Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Figure 1

Cardiac endothelial cells were isolated from P1 CD-1 mouse hearts by using the Neonatal Cardiac Endothelial Cell Isolation Kit, one LD Column with MidiMACS

^™

Separator, and one MS Column with MiniMACS™ Separator. The cells were fluorescently stained with CD90.2-APC (# 130-091-790) and CD31-PE (# 130-097-422) and analyzed by flow cytometry using the MACSQuant

^®

Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Figure 1

Cardiac endothelial cells were isolated from P1 CD-1 mouse hearts by using the Neonatal Cardiac Endothelial Cell Isolation Kit, one LD Column with MidiMACS

^™

Separator, and one MS Column with MiniMACS™ Separator. The cells were fluorescently stained with CD90.2-APC (# 130-091-790) and CD31-PE (# 130-097-422) and analyzed by flow cytometry using the MACSQuant

^®

Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Specifications

Specifications for Neonatal Cardiac Endothelial Cell Isolation Kit, mouse

Overview

The Neonatal Cardiac Endothelial Cell Isolation Kit, mouse has been developed for the two-step isolation of vital murine cardiac endothelial cells (P0-P3). Enriched cardiac endothelial cells are fully functional and can be used for various downstream applications.

Detailed product information

Background information

Besides cardiomyocytes and fibroblasts, endothelial cells represent the third most frequent cell population in the neonatal mouse heart. Endothelial cells form the inner layer of blood vessels and play a key role in vascular development, homeostasis and remodeling. The Neonatal Cardiac Endothelial Cell Isolation Kit, mouse has been designed for enrichment of cardiac endothelial cells from dissociated neonatal mouse hearts. The isolation of cardiac endothelial cells has successfully been tested using neonatal mouse hearts from postnatal day 0 to day 3 (P0-P3). For optimal use, the Neonatal Cardiac Endothelial Cell Isolation Kit should be used in combination with the Neonatal Heart Dissociation Kit, mouse and rat (# 130-098-373).

Columns

For the first magnetic separation (depletion): LD or autoMACS

^®

Columns. For the second magnetic separation (positive selection): MS or autoMACS Columns.

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Specifications for Neonatal Cardiac Endothelial Cell Isolation Kit, mouse

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Figure 1

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Specifications for Neonatal Cardiac Endothelial Cell Isolation Kit, mouse

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Detailed product information

Background information

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Neonatal Cardiac Endothelial Cell Isolation Kit, mouse

Neonatal Cardiac Endothelial Cell Isolation Kit, mouse

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