Neonatal Cardiomyocyte Isolation Kit, mouse

Name: Neonatal Cardiomyocyte Isolation Kit, mouse
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Neonatal Cardiomyocyte Isolation Kit, mouse

The Neonatal Cardiomyocyte Isolation Kit, mouse has been developed for the untouched isolation of vital murine cardiomyocytes (P0-P3). Enriched cardiomyocytes are fully functional and can be used for downstream applications.

Product data and images for Neonatal Cardiomyocyte Isolation Kit, mouse

Figures

Figure 1

Neonatal cardiomyocytes were isolated from P2 CD-1

^®

hearts using the Neonatal Heart Dissociation Kit, mouse and rat, the Neonatal Cardiomyocyte Isolation Kit, mouse, an MS Column, and a MiniMACS

^™

Separator. Subsequently, cells were fixed and stained using the Inside Stain Kit (# 130-090-477) and a FITC-conjugated antibody specific for cardiomyocytes (anti-α-actinin). Cells were analyzed by flow cytometry using the MACSQuant

^®

Analyzer. Cell debris were excluded from the analysis based on scatter signals.

Unseparated fraction

After separation

Figure 1

Neonatal cardiomyocytes were isolated from P2 CD-1

^®

hearts using the Neonatal Heart Dissociation Kit, mouse and rat, the Neonatal Cardiomyocyte Isolation Kit, mouse, an MS Column, and a MiniMACS

^™

Separator. Subsequently, cells were fixed and stained using the Inside Stain Kit (# 130-090-477) and a FITC-conjugated antibody specific for cardiomyocytes (anti-α-actinin). Cells were analyzed by flow cytometry using the MACSQuant

^®

Analyzer. Cell debris were excluded from the analysis based on scatter signals.

Figure 1

Neonatal cardiomyocytes were isolated from P2 CD-1

^®

hearts using the Neonatal Heart Dissociation Kit, mouse and rat, the Neonatal Cardiomyocyte Isolation Kit, mouse, an MS Column, and a MiniMACS

^™

Separator. Subsequently, cells were fixed and stained using the Inside Stain Kit (# 130-090-477) and a FITC-conjugated antibody specific for cardiomyocytes (anti-α-actinin). Cells were analyzed by flow cytometry using the MACSQuant

^®

Analyzer. Cell debris were excluded from the analysis based on scatter signals.

Specifications for Neonatal Cardiomyocyte Isolation Kit, mouse

Overview

The Neonatal Cardiomyocyte Isolation Kit, mouse has been developed for the untouched isolation of vital murine cardiomyocytes (P0-P3). Enriched cardiomyocytes are fully functional and can be used for downstream applications.

Detailed product information

Background information

One of the most used experimental model in cardiac research is the isolation of vital cardiac cells from neonatal hearts. Especially the isolation and subsequent culture of cardiomyocytes for biochemical, physiological, pharmacological and morphological studies has an high impact in the field of cardiovascular research. The Neonatal Cardiomyocyte Isolation Kit, mouse has been designed for the enrichment of untouched neonatal cardiomyocytes from dissociated neonatal mouse hearts. Non-cardiomyocytes are directly magnetically labeled by utilizing a cocktail of MicroBead-conjugated antibodies specific for non-cardiomyocytes. Isolation of pure unlabeled cardiomyocytes is achieved by depletion of magnetically labeled cells. The isolation of cardiomyocytes has been tested utilizing neonates aged from postnatal day 0 (P0) to postnatal day 3 (P3). For opimimal results, the Neonatal Heart Dissociation Kit has to be used prior cardiomyocyte isolation using this kit.

Applications

Enrichment of untouched cardiomyocytes from neonatal (P0- P3) mouse hearts.
Culture of enriched cardiomyocytes for biochemical, physiological, pharmacological, and morphological studies.

Columns

MS or autoMACS

^®

Columns.

References for Neonatal Cardiomyocyte Isolation Kit, mouse

Publications

Lakhal-Littleton, S. et al. (2015) Cardiac ferroportin regulates cellular iron homeostasis and is important for cardiac function. Proc. Natl. Acad. Sci. U.S.A. 112(10): 3164-3169
O' Meara, C. C. et al. (2015) Transcriptional reversion of cardiac myocyte fate during mammalian cardiac regeneration. Circ. Res. 116(5): 804-815
Trantidou, T. et al. (2015) Biorealistic cardiac cell culture platforms with integrated monitoring of extracellular action potentials. Sci Rep : 1-13

Data and images

Data and images for Neonatal Cardiomyocyte Isolation Kit, mouse

Figures

Figure 1

Neonatal cardiomyocytes were isolated from P2 CD-1

^®

hearts using the Neonatal Heart Dissociation Kit, mouse and rat, the Neonatal Cardiomyocyte Isolation Kit, mouse, an MS Column, and a MiniMACS

^™

Separator. Subsequently, cells were fixed and stained using the Inside Stain Kit (# 130-090-477) and a FITC-conjugated antibody specific for cardiomyocytes (anti-α-actinin). Cells were analyzed by flow cytometry using the MACSQuant

^®

Analyzer. Cell debris were excluded from the analysis based on scatter signals.

Unseparated fraction

After separation

Figure 1

Neonatal cardiomyocytes were isolated from P2 CD-1

^®

hearts using the Neonatal Heart Dissociation Kit, mouse and rat, the Neonatal Cardiomyocyte Isolation Kit, mouse, an MS Column, and a MiniMACS

^™

Separator. Subsequently, cells were fixed and stained using the Inside Stain Kit (# 130-090-477) and a FITC-conjugated antibody specific for cardiomyocytes (anti-α-actinin). Cells were analyzed by flow cytometry using the MACSQuant

^®

Analyzer. Cell debris were excluded from the analysis based on scatter signals.

Figure 1

Neonatal cardiomyocytes were isolated from P2 CD-1

^®

hearts using the Neonatal Heart Dissociation Kit, mouse and rat, the Neonatal Cardiomyocyte Isolation Kit, mouse, an MS Column, and a MiniMACS

^™

Separator. Subsequently, cells were fixed and stained using the Inside Stain Kit (# 130-090-477) and a FITC-conjugated antibody specific for cardiomyocytes (anti-α-actinin). Cells were analyzed by flow cytometry using the MACSQuant

^®

Analyzer. Cell debris were excluded from the analysis based on scatter signals.

Specifications

Specifications for Neonatal Cardiomyocyte Isolation Kit, mouse

Overview

The Neonatal Cardiomyocyte Isolation Kit, mouse has been developed for the untouched isolation of vital murine cardiomyocytes (P0-P3). Enriched cardiomyocytes are fully functional and can be used for downstream applications.

Detailed product information

Background information

One of the most used experimental model in cardiac research is the isolation of vital cardiac cells from neonatal hearts. Especially the isolation and subsequent culture of cardiomyocytes for biochemical, physiological, pharmacological and morphological studies has an high impact in the field of cardiovascular research. The Neonatal Cardiomyocyte Isolation Kit, mouse has been designed for the enrichment of untouched neonatal cardiomyocytes from dissociated neonatal mouse hearts. Non-cardiomyocytes are directly magnetically labeled by utilizing a cocktail of MicroBead-conjugated antibodies specific for non-cardiomyocytes. Isolation of pure unlabeled cardiomyocytes is achieved by depletion of magnetically labeled cells. The isolation of cardiomyocytes has been tested utilizing neonates aged from postnatal day 0 (P0) to postnatal day 3 (P3). For opimimal results, the Neonatal Heart Dissociation Kit has to be used prior cardiomyocyte isolation using this kit.

Applications

Enrichment of untouched cardiomyocytes from neonatal (P0- P3) mouse hearts.
Culture of enriched cardiomyocytes for biochemical, physiological, pharmacological, and morphological studies.

Columns

MS or autoMACS

^®

Columns.

References

References for Neonatal Cardiomyocyte Isolation Kit, mouse

Publications

Lakhal-Littleton, S. et al. (2015) Cardiac ferroportin regulates cellular iron homeostasis and is important for cardiac function. Proc. Natl. Acad. Sci. U.S.A. 112(10): 3164-3169
O' Meara, C. C. et al. (2015) Transcriptional reversion of cardiac myocyte fate during mammalian cardiac regeneration. Circ. Res. 116(5): 804-815
Trantidou, T. et al. (2015) Biorealistic cardiac cell culture platforms with integrated monitoring of extracellular action potentials. Sci Rep : 1-13

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Neonatal Cardiomyocyte Isolation Kit, mouse

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Product data and images for Neonatal Cardiomyocyte Isolation Kit, mouse

Figures

Figure 1

Figure 1

Figure 1

Specifications for Neonatal Cardiomyocyte Isolation Kit, mouse

Overview

Detailed product information

Background information

Applications

Columns

References for Neonatal Cardiomyocyte Isolation Kit, mouse

Publications

Data and images

Data and images for Neonatal Cardiomyocyte Isolation Kit, mouse

Figures

Figure 1

Figure 1

Figure 1

Specifications

Specifications for Neonatal Cardiomyocyte Isolation Kit, mouse

Overview

Detailed product information

Background information

Applications

Columns

References

References for Neonatal Cardiomyocyte Isolation Kit, mouse

Publications

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Neonatal Cardiomyocyte Isolation Kit, mouse

6 products available | view all view less

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Neonatal Cardiomyocyte Isolation Kit, mouse

Neonatal Cardiomyocyte Isolation Kit, mouse

Product data and images for Neonatal Cardiomyocyte Isolation Kit, mouse

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Figure 1