The IL-4 Secretion Assay was developed for the sensitive detection of mouse IL-4-secreting cells.

Data and images for Mouse IL-4 Secretion Assay – Detection Kit (PE)

Figures

Figure 1

BALB/c mice were immunized and the spleen cells restimulated with KLH (keyhole limpet hemocyanin). The responding cells were stained according to secretion of IL-4 using the Mouse IL-4 Secretion Assay - Detection Kit.
In the stimulated sample, 0.360% of all CD4
+
T cells secreted IL-4. In the unstimulated control sample, only 0.027% of all CD4
+
T cells secreted IL-4.
Stimulated sample
Unstimulated control
View details

Figure 1

BALB/c mice were immunized and the spleen cells restimulated with KLH (keyhole limpet hemocyanin). The responding cells were stained according to secretion of IL-4 using the Mouse IL-4 Secretion Assay - Detection Kit.
In the stimulated sample, 0.360% of all CD4
+
T cells secreted IL-4. In the unstimulated control sample, only 0.027% of all CD4
+
T cells secreted IL-4.
View details

Figure 1

BALB/c mice were immunized and the spleen cells restimulated with KLH (keyhole limpet hemocyanin). The responding cells were stained according to secretion of IL-4 using the Mouse IL-4 Secretion Assay - Detection Kit.
In the stimulated sample, 0.360% of all CD4
+
T cells secreted IL-4. In the unstimulated control sample, only 0.027% of all CD4
+
T cells secreted IL-4.

Specifications for Mouse IL-4 Secretion Assay – Detection Kit (PE)

Overview

The IL-4 Secretion Assay was developed for the sensitive detection of mouse IL-4-secreting cells.

Detailed product information

Background information

Interleukin-4 (IL-4) is predominantly secreted by activated CD4
+
memory and effector T
H
2 cells, basophils, and mast cells. It induces and supports humoral immune responses for the neutralization of extracellular pathogens. The T
H
2 types of immune mechanisms can also be involved in immunologic disorders, such as IgE-mediated allergy.

Downstream applications

The assay has been used for research on T
H
2 development and commitment
1,3
and for evaluation of IL-4 regulation and expression
2,4
.
The Mouse IL-4 Secretion Assay (PE) is suitable for two-color cytokine analysis in combination with the Mouse IFN-γ, IL-2, IL-5, or IL-10 Secretion Assay - Detection Kit (APC).

Resources for Mouse IL-4 Secretion Assay – Detection Kit (PE)

References for Mouse IL-4 Secretion Assay – Detection Kit (PE)

Publications

  1. Nakae, S. et al. (2012) Epithelial cell-derived IL-25, but not Th17 cell-derived IL-17 or IL-17F, is crucial for murine asthma. J. Immunol. 189(7): 3641-3652
  2. Avram, D. et al. (2018) Bcl11b is essential for licensing Th2 differentiation during helminth infection and allergic asthma. Nat Commun. 9(1): 1679
  3. Ouyang, W. et al. (2000) Stat6-independent GATA-3 autoactivation directs IL-4-independent Th2 development and commitment. Immunity 12: 27-37
  4. Hu-Li, J. et al. (2001) Regulation of expression of IL-4 alleles: analysis using a chimeric GFP/IL-4 gene. Immunity 14: 1-11
  5. Löhning, M. et al. (2003) Establishment of memory for IL-10 expression in developing T helper 2 cells requires repetitive IL-4 costimulation and does not impair proliferation. Proc. Natl. Acad. Sci. U.S.A. 100: 12307-12312
  6. Guo, L. et al. (2004) Probabilistic regulation of IL-4 production in Tʜ2 cells: accessibility at the Il4 locus. Immunity 20: 193-203
  7. Yang, J. Q. et al. (2003) Immunoregulatory role of CD1d in the hydrocarbon oil-induced model of lupus nephritis. J. Immunol. 171: 2142-2153

Related products for
Mouse IL-4 Secretion Assay – Detection Kit (PE)

3 products available