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Mouse spleen cells were incubated with or without PMA/ionomycin for 6 hours. After 2 hours brefeldin A was added. Cells were harvested, fixed, permeabilized, and intracellularly stained with Anti-IL-10 antibodies as well as with CD4-FITC and CD154 antibodies. Cells were analyzed by flow cytometry using the MACSQuant® Analyzer. Gating was performed according to forward scatter and side scatter properties of the cells.
Clone | JES5-16E3 |
---|---|
Clonality | monoclonal |
Isotype | rat IgG2b, rat IgG2bκ |
Isotype control | Isotype Control Antibody, rat IgG2b |
Host | rat |
Type of antibody | Primary antibodies, Functional-grade antibodies |
Species | mouse |
Antigen | IL-10 |
Alternative names of antigen | IL10, CSIF |
Molecular mass of antigen [kDa] | 19 |
Entrez Gene ID | 16153 |
RRID | AB_10828558, AB_2660624, AB_2660625, AB_2660626, AB_2660627, AB_2660628, AB_2660629 |
Clone | JES5-16E3 |
---|---|
Clonality | monoclonal |
Isotype | rat IgG2b, rat IgG2bκ |
Isotype control | Isotype Control Antibody, rat IgG2b |
Host | rat |
Type of antibody | Primary antibodies, Functional-grade antibodies |
Species | mouse |
Antigen | IL-10 |
Alternative names of antigen | IL10, CSIF |
Molecular mass of antigen [kDa] | 19 |
Entrez Gene ID | 16153 |
RRID | AB_10828558, AB_2660624, AB_2660625, AB_2660626, AB_2660627, AB_2660628, AB_2660629 |
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