Clone:
His51
Type of antibody:
Primary antibodies
Isotype:
mouse IgG2aκ
Applications:
FC, MICS, IF, IHC
Alternative names:
THY1, T25, Thy-1, Thy-1.2, Thy-1.1

Extended validation for CD90.1 Antibody, anti-mouse/rat

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with His51
REAL505++
REA838++
OX-7+
KW322+
Cells were incubated with an excess of purified unconjugated CD90.1 (His51) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Specifications for CD90.1 Antibody, anti-mouse/rat

Overview

The mouse monoclonal antibody His51 reacts with the rat CD90 (Thy-1) and mouse CD90.1 (Thy1.1) antigens, which belong to a GPI-anchored conserved membrane glycoprotein. In the mouse strains AKR/J, PL, and FVB/N, CD90.1 is a pan T cell marker and can be found on thymocytes, hematopoietic stem cells in the bone marrow, intra-epithelial cells (dendritic epidermal cells) in skin, and on neurons, such as retinal ganglion cells.
In the rat, the CD90 antigen is expressed on thymocytes, recent thymic emigrants, hematopoietic stem cells, on neurons such as retinal ganglion cells, and on other cell types. The antibody does not cross-react with CD90.2 (Thy1.2).

Alternative names

THY1, T25, Thy-1, Thy-1.2, Thy-1.1

Detailed product information

Technical specifications

CloneHis51
Clonalitymonoclonal
Isotypemouse IgG2aκ
Isotype controlIsotype Control Antibody, mouse IgG2a
Hostmouse
Type of antibodyPrimary antibodies
Speciesmouse, rat
AntigenCD90.1
Alternative names of antigenTHY1, T25, Thy-1, Thy-1.2, Thy-1.1
Molecular mass of antigen [kDa]13
Distribution of antigenendothelial cells, fibroblasts, neurons, T cells, stem cells, leukocytes, lymphocytes, cancer stem cells, hematopoietic stem and progenitor cells, leukemia cells, mesenchymal stem cells, ES and iPS cells, thymocytes, brain
Entrez Gene ID21838
RRIDAB_2659816, AB_2659817, AB_2659818, AB_2659819, AB_2659820, AB_2659821, AB_2659822, AB_2659823, AB_10829750, AB_2659815

Resources for CD90.1 Antibody, anti-mouse/rat

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for CD90.1 Antibody, anti-mouse/rat

Publications

  1. Nikoozad, Z. et al. (2014) Comparison of the liver function and hepatic specific genes expression in cultured mesenchymal stem cells and hepatocytes. Iran J Basic Med Sci 17(1): 27-33

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