The CD4
+
Dendritic Cell Isolation Kit is an indirect magnetic labeling system designed to isolate CD4
+
DCs from single-cell suspensions of splenocytes.

Data and images for
CD4
+
Dendritic Cell Isolation Kit
, mouse

Figures

Figure 1

Isolation of CD4
+
DCs from a mouse spleen cell suspension using the CD4
+
Dendritic Cell Isolation Kit, an LD and two MS Columns, a MidiMACS™ and a MiniMACS™ Separator.
Spleen cells before separation
Enriched CD4
+
CD11c
+
DCs
Isolated CD4
+
CD11c
+
DCs

Specifications for
CD4
+
Dendritic Cell Isolation Kit
, mouse

Overview

The CD4
+
Dendritic Cell Isolation Kit is an indirect magnetic labeling system designed to isolate CD4
+
DCs from single-cell suspensions of splenocytes.

Detailed product information

Background information

CD4
+
DCs coexpress CD11c, CD11b, MHC class II, CD40, CD80, CD86 and are negative for CD8 and CD205. They represent the major population of DCs in spleen and comprise only a small population in peripheral lymph nodes (56% and 4% of all CD11c
+
DCs, respectively). The isolation of CD4
+
DCs is performed in two steps.

Detailed separation procedure

First, DCs are enriched by depletion of T cells, NK cells, B cells, and granulocytes with a cocktail of biotin-conjugated antibodies and Anti-Biotin MicroBeads. Subsequently, CD4
+
DCs are positively selected from the enriched DC fraction with CD4 MicroBeads.

Downstream applications

CD4
+
DCs isolated using the CD4
+
Dendritic Cell Isolation Kit are suitable to analyze antigen uptake, antigen processing, T cell activation or T cell tolerance induction, cross-priming of cytotoxic T cells, and T cell polarization by CD4
+
DCs.

Columns

For the first magnetic separation (depletion): LD or autoMACS
®
Columns. For the second magnetic separation (positive selection): MS or autoMACS Columns.

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CD4
+
Dendritic Cell Isolation Kit
, mouse

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+
Dendritic Cell Isolation Kit
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