CD4
⁺
Dendritic Cell Isolation Kit
, mouse

Name: CD4+ Dendritic Cell Isolation Kit, mouse
Availability: InStock

CD4
⁺
Dendritic Cell Isolation Kit
, mouse

The CD4

⁺

Dendritic Cell Isolation Kit is an indirect magnetic labeling system designed to isolate CD4

⁺

DCs from single-cell suspensions of splenocytes.

Data and images
Specifications

Product data and images for CD4
⁺
Dendritic Cell Isolation Kit
, mouse

Figures

Figure 1

Isolation of CD4

⁺

DCs from a mouse spleen cell suspension using the CD4

⁺

Dendritic Cell Isolation Kit, an LD and two MS Columns, a MidiMACS™ and a MiniMACS™ Separator.

Spleen cells before separation	Enriched CD4 ⁺ CD11c ⁺ DCs
View details CD4 ⁺ Dendritic Cell Isolation Kit , mouse Figure 1 Isolation of CD4 ⁺ DCs from a mouse spleen cell suspension using the CD4 ⁺ Dendritic Cell Isolation Kit, an LD and two MS Columns, a MidiMACS™ and a MiniMACS™ Separator.	View details CD4 ⁺ Dendritic Cell Isolation Kit , mouse Figure 1 Isolation of CD4 ⁺ DCs from a mouse spleen cell suspension using the CD4 ⁺ Dendritic Cell Isolation Kit, an LD and two MS Columns, a MidiMACS™ and a MiniMACS™ Separator.
Isolated CD4 ⁺ CD11c ⁺ DCs
View details CD4 ⁺ Dendritic Cell Isolation Kit , mouse Figure 1 Isolation of CD4 ⁺ DCs from a mouse spleen cell suspension using the CD4 ⁺ Dendritic Cell Isolation Kit, an LD and two MS Columns, a MidiMACS™ and a MiniMACS™ Separator.

Specifications for CD4
⁺
Dendritic Cell Isolation Kit
, mouse

Overview

The CD4

⁺

Dendritic Cell Isolation Kit is an indirect magnetic labeling system designed to isolate CD4

⁺

DCs from single-cell suspensions of splenocytes.

Detailed product information

Background information

CD4

⁺

DCs coexpress CD11c, CD11b, MHC class II, CD40, CD80, CD86 and are negative for CD8 and CD205. They represent the major population of DCs in spleen and comprise only a small population in peripheral lymph nodes (56% and 4% of all CD11c

⁺

DCs, respectively). The isolation of CD4

⁺

DCs is performed in two steps.

Detailed separation procedure

First, DCs are enriched by depletion of T cells, NK cells, B cells, and granulocytes with a cocktail of biotin-conjugated antibodies and Anti-Biotin MicroBeads. Subsequently, CD4

⁺

DCs are positively selected from the enriched DC fraction with CD4 MicroBeads.

Downstream applications

CD4

⁺

DCs isolated using the CD4

⁺

Dendritic Cell Isolation Kit are suitable to analyze antigen uptake, antigen processing, T cell activation or T cell tolerance induction, cross-priming of cytotoxic T cells, and T cell polarization by CD4

⁺

DCs.

Columns

For the first magnetic separation (depletion): LD or autoMACS

^®

Columns. For the second magnetic separation (positive selection): MS or autoMACS Columns.

Data and images