CYTO Virtual 2020

Despite its critical role for the production and release testing of cell products in modern cell therapies, flow cytometry remains a notoriously variable technique. However, reproducibility and consistency are of key concern when monitoring cell production processes and releases of products, such as CAR T cell therapies. Therefore, it is essential that in-process control and quality control (IPC/QC) using flow cytometry fulfill essential standardization criteria. 

During this pre-recorded tutorial, you will learn about:

• antibody-related reproducibility crisis and implications for antibody-based quality control in cell manufacturing
• advances in recombinant antibody technology and opportunities for flow cytometry assays
• introduction to REAfinity™ Recombinant Antibodies and exemplary data in cell manufacturing assays

Single-cell genomics and other next generation sequencing applications depend strongly on adequate upstream sample preparation. However, harsh conditions during those workflows can easily affect your results in a variety of ways. This is especially troublesome if you are interested in genes related to cellular stress, activation, or metabolism. How can you guarantee you’re using only non-stressed, viable target cells in high purities in your genomics experiments?

During this pre-recorded tutorial, you will learn about:

• typical mistakes in sample preparation prior to NGS analysis
• a better way to design your cell sorting steps
• how other scientists have improved their results