Clone:
4B4-1
Type of antibody:
Primary antibodies
Isotype:
mouse IgG1κ
Applications:
FC, MICS, IF, IHC
Alternative names:
TNFRSF9, 4-1BB, CDw137, ILA

Extended validation for CD137 Antibody, anti-human

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with 4B4-1
REA765++
Cells were incubated with an excess of purified unconjugated CD137 (4B4-1) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for CD137. Human peripheral blood mononuclear cells (PBMCs) stimulated with CD3/CD28 antibodies (T Cell TransAct™) for 16 hours were stained with CD137 antibodies and with a suitable counterstaining. As a control, CD137 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. CD4+ cells were pregated for the analysis. Cell debris and cell doublets were excluded from the analysis based on scatter signals. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD137. Human peripheral blood mononuclear cells (PBMCs) stimulated with CD3/CD28 antibodies (T Cell TransAct™) for 16 hours were stained with CD137 antibodies and with a suitable counterstaining. As a control, CD137 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. CD4+ cells were pregated for the analysis. Cell debris and cell doublets were excluded from the analysis based on scatter signals. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD137. Human peripheral blood mononuclear cells (PBMCs) stimulated with CD3/CD28 antibodies (T Cell TransAct™) for 16 hours were stained with CD137 antibodies and with a suitable counterstaining. As a control, CD137 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. CD4+ cells were pregated for the analysis. Cell debris and cell doublets were excluded from the analysis based on scatter signals. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD137. Human peripheral blood mononuclear cells (PBMCs) stimulated with CD3/CD28 antibodies (T Cell TransAct™) for 16 hours were stained with CD137 antibodies and with a suitable counterstaining. As a control, CD137 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. CD4+ cells were pregated for the analysis. Cell debris and cell doublets were excluded from the analysis based on scatter signals. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for CD137. Human peripheral blood mononuclear cells (PBMCs) stimulated with CD3/CD28 antibodies (T Cell TransAct™) for 16 hours were stained with CD137 antibodies and with a suitable counterstaining. As a control, CD137 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. CD4+ cells were pregated for the analysis. Cell debris and cell doublets were excluded from the analysis based on scatter signals. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using CD137 (4B4-1). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD137 (4B4-1). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using CD137 (4B4-1). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD137 Antibody, anti-human

Overview

This antibody recognizes the CD137 (4-1BB) antigen, a 30 kDa glycoprotein of the tumor necrosis factor (TNF) receptor superfamily. CD137 is mainly expressed on activated CD4
+
and CD8
+
T cells, activated B cells, and natural killer cells, but can also be found on resting monocytes and dendritic cells.
As a costimulatory molecule it is involved in the activation and survival of CD4
+
or CD8
+
T cells, and NK cells. CD137 has been described to be a suitable marker for antigen-specific activation of human CD8
+
T cells, as it is not expressed on resting CD8
+
T cells and its expression is reliably induced after 24 hours of stimulation.

Alternative names

TNFRSF9, 4-1BB, CDw137, ILA

Detailed product information

Technical specifications

Clone4B4-1
Clonalitymonoclonal
Isotypemouse IgG1κ
Isotype controlIsotype Control Antibody, mouse IgG1
Hostmouse
Type of antibodyPrimary antibodies
Specieshuman, non-human primate
Cross-reactivity
rhesus monkey (
Macaca mulatta
)
,
cynomolgus monkey (
Macaca fascicularis
)
AntigenCD137
Alternative names of antigenTNFRSF9, 4-1BB, CDw137, ILA
Molecular mass of antigen [kDa]25
Distribution of antigenB cells, monocytes, T cells, dendritic cells, epithelial cells, fibroblasts, macrophages
Entrez Gene ID3604
RRIDAB_2783946, AB_2783945, AB_2783944, AB_2751952, AB_2751905, AB_2751953, AB_2751906, AB_2783947

Resources for CD137 Antibody, anti-human

References for CD137 Antibody, anti-human

Publications

  1. Wolfl, M. et al. (2007)
    Activation-induced expression of CD137 permits detection, isolation, and expansion of the full repertoire of CD8
    +
    T cells responding to antigen without requiring knowledge of epitope specificities.
    Blood 110: 201-210
  2. Wehler, T. C. et al. (2007) Targeting the activation-induced antigen CD137 can selectively deplete alloreactive T cells from antileukemic and antitumor donor T-cell lines. Blood 109: 365-373

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