The Adult Brain Dissociation Kit has been designed for the dissociation of rodent neural tissue older than P7 for the subsequent isolation of neurons, astrocytes, or oligodendrocytes.

Specifications for Adult Brain Dissociation Kit, mouse and rat

Overview

The Adult Brain Dissociation Kit has been designed for the dissociation of rodent neural tissue older than P7 for the subsequent isolation of neurons, astrocytes, or oligodendrocytes.

Detailed product information

Background information

Brain tissue from adult mice or rats older than P7 is dissociated into single-cell suspensions. The extracellular matrix is enzymatically digested using the kit components, while the gentleMACS™ Dissociator with Heaters is used for the mechanical dissociation steps during the on-instrument enzyme incubation. After the dissociation, the Debris Removal Solution is used for the elimination of debris, and the Red Blood Cell Removal Solution is used for the removal of erythrocytes.
The single-cell suspension may be analyzed for phenotype distributions, and other functional, genetic, or proteomic studies can be performed. Furthermore, dissociated cells can be cultured, or neurons, astrocytes, or oligodendrocytes can be isolated using MACS
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Technology.

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Single Cell isolation from Adult Mouse Brain

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Adult Brain Dissociation Kit, mouse and rat (130-107-677)

We use in our lab this Kit combined with MACS gentle dissociator to dissociate the adult brain from the mouse. The protocol is highly optimized by miltenyi Biotech for mouse brain that yileds high amount single cells from the mouse brain. We use routinely in our lab that yield consistent reproducible result across different hands.

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