CD45 (TIL) MicroBeads, human

CD45 (TIL) MicroBeads, human have been optimized for the isolation of tumor-infiltrating leukocytes from human tumors as well as  xenotransplanted tumors with a humanized immune system.

Data and images for CD45 (TIL) MicroBeads, human

Figures

Figure 1

A human colorectal carcinoma sample was dissociated using the gentleMACS™ Octo Dissociator with Heaters in combination with the Tumor Dissociation Kit, human (# 130-095-929). CD45
+
TILs were isolated from the single-cell suspension using CD45 (TIL) MicroBeads, an LS Column, and a QuadroMACS™ Separator.
Cells were fluorescently stained with CD45-PE-Vio
®
770 and analyzed by flow cytometry using the MACSQuant
®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
Before separation
CD45
+
cells
View details

Figure 1

A human colorectal carcinoma sample was dissociated using the gentleMACS™ Octo Dissociator with Heaters in combination with the Tumor Dissociation Kit, human (# 130-095-929). CD45
+
TILs were isolated from the single-cell suspension using CD45 (TIL) MicroBeads, an LS Column, and a QuadroMACS™ Separator.
Cells were fluorescently stained with CD45-PE-Vio
®
770 and analyzed by flow cytometry using the MACSQuant
®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
View details

Figure 1

A human colorectal carcinoma sample was dissociated using the gentleMACS™ Octo Dissociator with Heaters in combination with the Tumor Dissociation Kit, human (# 130-095-929). CD45
+
TILs were isolated from the single-cell suspension using CD45 (TIL) MicroBeads, an LS Column, and a QuadroMACS™ Separator.
Cells were fluorescently stained with CD45-PE-Vio
®
770 and analyzed by flow cytometry using the MACSQuant
®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Specifications for CD45 (TIL) MicroBeads, human

Overview

CD45 (TIL) MicroBeads, human have been optimized for the isolation of tumor-infiltrating leukocytes from human tumors as well as  xenotransplanted tumors with a humanized immune system.

Detailed product information

Background information

CD45 (TIL) MicroBeads, human enable a positive selection of CD45
+
tumor-infiltrating leukocytes (TILs) specifically from human tumors as well as xenotransplanted tumors with a humanized immune system. The CD45 antigen is expressed on all cells of hematopoietic origin except erythrocytes and platelets.

Columns

MS, LS, autoMACS
®
Columns, or Multi-24 Column Blocks

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CD45 (TIL) MicroBeads, human

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