Miltenyi Biotec-tested panel 13 (MBTP 13)
This application protocol describes the flow cytometric analysis of monocytes after spleen dissociation from healthy C57BL/6 mice. Mouse spleens are easily dissociated using the gentleMACS™ Dissociators to quickly obtain viable single cell suspensions ready for flow cytometric analysis.
|Specificity||Clone||Purpose||Fluorochrome||Detection filter (nm) (laser)|
|Viobility™ 405/520 Fixable Dye||-||Viability||-||525/50 (violet)|
|F4/80||REA126||Cell type exclusion||FITC||525/50 (blue)|
|CD192 (CCR2)||REA538||Splenic monocytes||PE||585/40 (blue)|
|CD3e||REA606||Lineage||PE-Vio® 615||655-730 (blue)|
|CD19||REA747||Lineage||PE-Vio 615||655-730 (blue)|
|CD335 (NKp46)||REA815||Lineage||PE-Vio 615||655-730 (blue)|
|Ly-6C||REA796||Monocytes||PE-Vio 770||750 LP (blue)|
|CD45||REA737||Leukocytes||APC-Vio 770||750 LP (red)|
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