MBTP 24: Immunophenotyping of innate lymphoid cell 2 (ILC2) subsets from mouse mesentery and small intestine using flow cytometry

Miltenyi Biotec-tested panel 24 (MBTP 24)

This application protocol describes the flow cytometric analysis of ILC2 subsets after dissociation of mesentery and small intestine from healthy C57BL/6 mice. Mouse mesentery and small intestine are easily dissociated using the gentleMACS™ Dissociators to quickly obtain viable single cell suspensions ready for downstream applications, such as flow cytometric analysis.

Protocol

Materials

Matching products: