Products and Services - MACS Sample Preparation - Sample dissociation - Tissue Dissociation Kits - Brain Tumor Dissociation Kit

Brain Tumor Dissociation Kit

Overview

The Brain Tumor Dissociation Kits have been developed for the gentle and effective generation of single-cell suspensions from human and mouse brain tumors, and can be used in combination with the gentleMACS™ Dissociator and the gentleMACS Octo Dissociator. In less than an hour, the gentle enzymatic dissociation procedure efficiently yields high numbers of viable cells. Dissociated cells are immediately ready for further in vitro or in vivo applications such as cell culture and expansion, flow cytometry, fluorescence microscopy, and molecular applications, or for cell separations using MACS® Technology.

Details

Background information

The quality of starting material is important for any experiment, and a good single-cell suspension is a prerequisite for many downstream applications. Human brain tumor samples can be effectively dissociated into single-cell suspensions using the Brain Tumor Dissociation Kit1. The procotol was developed using human brain tumors, mainly glioblastomas. It can also be transferred to mouse samples.
The Brain Tumor Dissociation Kits (T) and (P) are based on trypsin and papain respectively. Some antigen epitopes are degraded by papain, others by trypsin. The kit based on papain is best suited for use with CD133 MicroBeads, CD11b (Microglia) MicroBeads and Anti-A2B5 MicroBeads, whereas, for example, the Anti-GLAST (ACSA-1) MicroBead Kit requires the use of the trypsin-based kit.
Please see Figure 1 in the gallery for further information with regard to epitope sensitivity and kit selection.

Gallery

Figure 1

Figure 1
Antigen compatibility of Dissociation Kits. For many applications, including MACS Cell Separation, it is crucial to conserve the integrity of particular cell surface epitopes during tissue dissociation. The table shows which kits conserve which specific epitopes. Epitope sensitivities have been tested using Miltenyi Biotec's antibodies if available.
Antigen compatibility of Dissociation Kits. For many applications, including MACS Cell Separation, it is crucial to conserve the integrity of particular cell surface epitopes during tissue dissociation. The table shows which kits conserve which specific epitopes. Epitope sensitivities have been tested using Miltenyi Biotec's antibodies if available.

Related Items

Library

Selected references

  1. Környei et al. (2007) FASEB J. 21: 2496–2509.

Brochures and posters

Neuroscience research brochure [PDF, 1.7 MB, EN]

Scientific posters

Product Order no. Price

Brain Tumor Dissociation Kit (P)

Capacity: for 25 preparations
 
Data sheet
130-095-942 $395.00 cart

Brain Tumor Dissociation Kit (T)

Capacity: for 25 preparations
 
Data sheet
130-095-939 $395.00 cart

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Miltenyi Biotec Inc.
Phone: +1 800 FOR MACS
Fax:+1 530 745 2806
macs@miltenyibiotec.com
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