Products and Services - MACS Sample Preparation - Applications and protocols

Applications and protocols

Keep up-to-date and browse our growing collection of protocols and videos to find everything you need to know about our sample preparation tools and never liked to ask.

Customer protocols

Below we have listed all protocols that have been developed and submitted by our customers*:

 

Generation of single-cell suspensions

Dissociation of human breast tumor to isolate cancer stem cells

Dissociation of human pancreatic tumor to quantify myeloid-derived suppressor cells

Dissociation of mouse lung to isolate endothelial cells

Dissociation of mouse kidney to isloate bone-marrow derived inflammatory cells

Dissociation of human melanoma to isolate tumor-infiltrating lymphocytes
Dissociation of human melanoma to isolate cancer stem cells
Dissociation of human synovial membrane to isolate T cells
Dissociation of human cervical explants to isolate T cells
Dissociation of human decidual tissue to isolate mononuclear cells
Dissociation of human adipose tissue to isolate mononuclear cells
Dissociation of human nasal mucosa to prepare single-cell suspension
Dissociation of human tonsil to isolate  mononuclear cells
Dissociation of mouse thymus to isolate thymic epithelial cells
Dissociation of human ovarian tumors to isolate cancer cells
Dissociation of human head and neck tumor to isolate tumor-infiltrating lymphocytes
Dissociation of mouse brain to isolate microglia
Dissociation of mouse spleen to isolate T cells
Dissociation of mouse ear to isolate T cells
Dissociation of mouse embryos to generate mouse embryonic fibroblasts
Dissociation of mouse embryos to isolate lymphatic endothelial cells
Dissociation of mouse vaginal tissue to isolate T cells
Dissociation of mouse glioblastoma to isolate CNS-infiltrating lymphocytes
Dissociation of bovine retina to isolate retinal endothelial cells
Dissociation of human ovarian tumor to isolate tumor stem cells

Dissociation of human dental pulp to isolate mesenchymal stem cells

Dissociation of zebrafish embryos to isolate neurons
Dissociation of grafted colon tumor to isolate tumor cells
Dissociation of grafted glioblastoma to isolate tumor cells
Dissociation of mouse adipose tissue to isolate adipocytes
Dissociation of neonatal rat hearts to isolate neonatal cardiomyocytes
Dissociation of neonatal mouse hearts to isolate neonatal cardiomyocytes
Dissociation of neonatal rat hearts to isolate ventricular myocytes

 

Isolation of pathogens

Isolation of Leishmania major from mouse skin
Isolation of Citrobacter rodentium from mouse spleen and liver
Isolation of Mycobacterium tuberculosis from rat lung
Isolation of Staphylococcus aureus from mouse kidney
Isolation of respiratory syncytial virus from mouse lung
Isolation of Acinetobacter baumannii from mouse lung and spleen
Isolation of colonic bacteria from mouse spleen, liver, and lymph nodes
Isolation of Influenza A from mouse lung
Isolation of Influenza H1N1 from mouse lung

Extraction of nucleic acids, proteins, and cell organelles

Homogenization of potatos for DNA extraction
Homogenization of zebrafish organs for protein and RNA extraction
Homogenization of human tumor for generation of autologous tumor lysates
Preparation of nuclei from human tumor samples
Measurement of intracellular kinase activity in different lung cell subtypes
Preparation of human tumor samples for a dendritic cell-based vaccine 

 

*Please note that Miltenyi Biotec has not subjected customer-submitted protocols to rigorous testing

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Neonatal heart dissociation

Neonatal heart dissociation

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